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Determination of peptide profile consistency and safety of collagen hydrolysates as quality attributes

dc.creatorLópez-Morales, Carlos A.es_ES
dc.creatorVázquez-Leyva, Saides_ES
dc.creatorVallejo-Castillo, Luises_ES
dc.creatorCarballo-Uicab, Gregorioes_ES
dc.creatorMuñoz-García, Lesliees_ES
dc.creatorHerbert-Pucheta, José Enriquees_ES
dc.creatorZepeda-Vallejo, L. Gerardoes_ES
dc.creatorVelasco-Velázquez, Marcoes_ES
dc.creatorPavón, Lenines_ES
dc.creatorPérez-Tapia, Sonia M.es_ES
dc.creatorMedina-Rivero, Emilioes_ES
dc.date2019
dc.date.accessioned2022-12-08T18:11:14Z
dc.date.available2022-12-08T18:11:14Z
dc.date.issued2019
dc.identifierJC24NC22es_ES
dc.identifier.issn0022-1147
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/7647
dc.identifier.urihttps://doi.org/10.1111/1750-3841.14466
dc.descriptionCollagen hydrolysates are dietary supplements used for nutritional and medical purposes. They are complex mixtures of low-molecular-weight peptides obtained from the enzymatic hydrolysis of collagen, which provide intrinsic batch-to-batch heterogeneity. In consequence, the quality of these products, which is related to the reproducibility of their mass distribution pattern, should be addressed. Here, we propose an analytical approach to determine the peptide pattern as a quality attribute of Colagenart®, a product containing collagen hydrolysate. In addition, we evaluated the safety by measuring the viability of two cell lines exposed to the product. The consistency of peptide distribution was determined using Size Exclusion Chromatography (SEC), Mass Spectrometry coupled to a reversed phase UPLC system (MS-RP-UPLC), and Shaped-pulse off-resonance water-presaturation proton nuclear magnetic resonance spectrometry [1 Hwater_presat NMR]. The mass distribution pattern determined by SEC was in a range from 1.35 to 17 kDa, and from 2 to 14 kDa by MS-RP-UPLC. [1 Hwater_presat NMR] showed the detailed spin-systems of the collagen hydrolysates components by global assignment of backbone Hα and NH, as well as side-chain proton resonances. Additionally, short-range intraresidue connectivity pathways of identified spin-regions were obtained by a 2D homonuclear shift correlation Shaped-pulse solvent suppression COSY scheme. Safety analysis of Colagenart® was evaluated in CaCo-2 and HepG2 cells at 2.5 and 25 μg/mL and no negative effects were observed. The results demonstrated batch-to-batch reproducibility, which evinces the utility of this approach to establish the consistency of the quality attributes of collagen hydrolysates. PRACTICAL APPLICATION: We propose state-of-the art analytical methodologies (SEC, MS, and NMR) to evaluate peptide profile and composition of collagen hydrolysates as quality attributes. These methodologies are suitable to be implemented for quality control purposes.es_ES
dc.formatPDFes_ES
dc.language.isoenges_ES
dc.publisherWiley on behalf of the Institute of Food Technologistses_ES
dc.relation84(3)430-439
dc.rightsAcceso Cerradoes_ES
dc.titleDetermination of peptide profile consistency and safety of collagen hydrolysates as quality attributeses_ES
dc.typeArtículoes_ES
dc.contributor.affiliationUnidad de Desarrollo e Investigación en Bioprocesos (UDIBI), Escuela Nacional de Ciencias Biológicas, Insto. Politécnico Nacional, Ciudad de México, 11340, México.
dc.contributor.emailemilio.medina@udibi.com.mx (Emilio Medina-Rivero)
dc.relation.jnabreviadoJ FOOD SCI
dc.relation.journalJournal of Food Science
dc.identifier.placeEstados Unidos
dc.date.published2019
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñiz
dc.identifier.eissn1750-3841
dc.identifier.doi10.1111/1750-3841.14466
dc.subject.kwCollagen hydrolysates
dc.subject.kwIon mobility mass spectrometry
dc.subject.kwNuclear magnetic resonance
dc.subject.kwQuality control
dc.subject.kwSize exclusion chromatography


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