Antinociceptive effects of Raphanus sativus sprouts involve the opioid and 5-HT1A serotonin receptors, cAMP/cGMP pathways, and the central activity of sulforaphane

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dc.contributor.affiliationLaboratorio de Neurofarmacología de Productos Naturales. Dirección de Investigaciones en Neurociencias, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz. Calz, México-Xochimilco 101, Col. San Lorenzo Huipulco, Tlalpan, 14370, Ciudad de México, Mexico
dc.contributor.emailevag@imp.edu.mx, msoto@colpos.mx
dc.creatorHernández-Sánchez, Laura Yunuen
dc.creatorGonzález-Trujano, María Eva
dc.creatorMoreno, Diego A.
dc.creatorMartínez-Vargas, David
dc.creatorVibrans, Heike
dc.creatorHernandez-Leon, Alberto
dc.creatorDorazco-González, Alejandro
dc.creatorPellicer, Francisco
dc.creatorSoto-Hernández, Marcos
dc.creator.identificadorhttps://orcid.org/0000-0003-4823-5928 (Hernández-Sánchez, Laura Yunuen)
dc.creator.identificadorhttps://orcid.org/0000-0002-4399-1508 (González-Trujano, María Eva)
dc.creator.identificadorhttps://orcid.org/0000-0002-6547-8764 (Moreno, Diego A.)
dc.creator.identificadorhttps://orcid.org/0000-0002-6392-1670 (Martínez-Vargas, David)
dc.creator.identificadorhttps://orcid.org/0000-0002-1800-4320 (Vibrans, Heike)
dc.creator.identificadorhttps://orcid.org/0000-0002-6864-6735 (Hernandez-Leon, Alberto)
dc.creator.identificadorhttps://orcid.org/0000-0002-7056-4237 (Dorazco-González, Alejandro)
dc.date2024
dc.date.accessioned2026-06-17T17:12:51Z
dc.date.issued2024
dc.date.published2024
dc.descriptionRaphanus sativus L. cv. Sango, commonly known as red radish, is widely consumed around the world as a vegetable, but its benefit in pain relief is not sufficiently investigated. This study aimed to evaluate the antinociceptive effects of R. sativus and a possible mechanism of action. An aqueous extract of R. sativus sprouts (AERSS) was investigated by parenteral (10, 30, and 100 mg kg-1, i.p.) and enteral (500 mg kg-1, p.o.) administration in the neurogenic and inflammatory phases of the formalin test, where gastric damage was also evaluated as a possible adverse effect. Ketorolac (5 mg kg-1, i.p.) was used as the reference drug. Endogenous opioid and 5-HT1A serotonin receptors, as well as the cAMP/NO-cGMP pathways, were explored in the study of a possible mechanism of action by using their corresponding antagonists: naloxone, 1 mg kg-1, i.p., WAY100635, 1 mg kg-1, i.p., and enzymatic activators or inhibitors, respectively. Sulforaphane (SFN), a known bioactive metabolite, was analyzed using electroencephalography (EEG) to evidence its central involvement. A significant and dose-dependent antinociceptive activity was observed with the AERSS resembling the antinociceptive effect of the reference drug, with an equivalent significant response with a dose of 500 mg kg-1, p.o. without causing gastric damage. The participation of the endogenous opioid and 5-HT1A serotonin receptors at central and peripheral levels was also observed, with a differential participation of cAMP/NO-cGMP. SFN as one metabolite produced significant changes in the EEG analysis, reinforcing its effects on the CNS. Our preclinical evidence supports the benefits of consuming Raphanus sativus cv. Sango sprouts for pain relief.
dc.formatPDF
dc.identifierJC07NC24
dc.identifier.doi10.1039/d3fo05229j
dc.identifier.eissn2042-650X
dc.identifier.issn2042-6496
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñiz
dc.identifier.placeInglaterra
dc.identifier.urihttps://repositorio.inprf.gob.mx/handle/123456789/111
dc.identifier.urihttps://doi.org/10.1039/d3fo05229j
dc.language.isoeng
dc.publisherRoyal Society of Chemistry
dc.relation15(9):4773-4784
dc.relation.jnabreviadoFOOD FUNCT
dc.relation.journalFood & Function
dc.rightsAcceso Cerrado
dc.titleAntinociceptive effects of Raphanus sativus sprouts involve the opioid and 5-HT1A serotonin receptors, cAMP/cGMP pathways, and the central activity of sulforaphane
dc.typeArtículo

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