Activity and expression of enkephalinase and aminopeptidase N in regions of the mesocorticolimbic system are selectively modified by acute ethanol administration

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dc.contributor.affiliationDepartamento de Neuroquímica, Subdirección de Investigaciones Clínicas, Instituto Nacional de Psiquiatría Ramón de la Fuente, Calzada México-Xochimilco 101, Col. San Lorenzo Huipulco, 14370, México, DF, Mexico.es_ES
dc.creatorMorales-Mulia, Marcela
dc.creatorGortari, Patricia de
dc.creatorAmaya, María-Isabel
dc.creatorMéndez, Milagros
dc.date.accessioned2017-06-30T02:06:11Z
dc.date.accessioned2026-03-27T14:47:09Z
dc.date.available2017-06-30T02:06:11Z
dc.date.issued2012es_ES
dc.date.published2012es_ES
dc.description.abstractotrodiomaOpioid peptides play a key role in ethanol reinforcement and alcohol drinking behavior. However, regulation of opioid levels by peptidase-degrading activities in ethanol's actions in brain is still unclear. The aim of this work was to study the acute effects of ethanol (2.5 g-kg) on enkephalinase (NEP) and aminopeptidase N (APN) activities and expression in regions of the mesocorticolimbic system, as well as on corticosterone levels in serum for up to 24 h after administration. Enzymatic activities were measured by fluorometric assays, mRNA's expression by reverse transcriptase polymerase chain reaction (RT-PCR) and corticosterone levels by radioimmunoassay. Acute ethanol administration modified peptidase activity and expression with different kinetics. Ethanol induced a transitory increase and decrease in NEP and APN activities in the frontal cortex (FC) and ventral tegmental area (VTA), whereas only increases in these activities were observed in the nucleus accumbens (NAcc). Ethanol induced an increase in NEP mRNA in the FC and decreases in APN mRNA in the FC and NAcc. In contrast, ethanol produced biphasic effects on both enzymes expression in the VTA. Corticosterone levels were not changed by ethanol. Our results suggest that NEP and APN could play a main role in ethanol reinforcement through regulation of opioid levels in mesolimbic areas.es_ES
dc.description.monthEnees_ES
dc.identifier1529es_ES
dc.identifier.citationJuan Carlos Bautista Ramírezes_ES
dc.identifier.doi10.1007/s12031-011-9623-2es_ES
dc.identifier.eissn1559-1166es_ES
dc.identifier.issn0895-8696es_ES
dc.identifier.numero1es_ES
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñizes_ES
dc.identifier.paginacion58-67es_ES
dc.identifier.placeEstados unidoses_ES
dc.identifier.urihttps://doi.org/10.1007/s12031-011-9623-2es_ES
dc.identifier.urihttps://repositorio.inprf.gob.mx/handle/123456789/6207
dc.identifier.volumen46es_ES
dc.language.isoenges_ES
dc.relation46 (1) 58-67 p.es_ES
dc.relationversión del editores_ES
dc.relation.jnabreviadoJ MOL NEUROSCIes_ES
dc.relation.journalJournal of Molecular Neurosciencees_ES
dc.rightsacceso cerradoes_ES
dc.subject.koAcute Diseasees_ES
dc.subject.koAlcohol Drinkinges_ES
dc.subject.kometabolismes_ES
dc.subject.koAlcohol-Induced Disorders, Nervous Systemes_ES
dc.subject.kometabolismes_ES
dc.subject.koAnimalses_ES
dc.subject.koAntigens, CD13es_ES
dc.subject.kobiosynthesises_ES
dc.subject.koAntigens, CD13es_ES
dc.subject.kogeneticses_ES
dc.subject.koEthanoles_ES
dc.subject.kotoxicityes_ES
dc.subject.koLimbic Systemes_ES
dc.subject.kodrug effectses_ES
dc.subject.koLimbic Systemes_ES
dc.subject.koenzymologyes_ES
dc.subject.koMalees_ES
dc.subject.koNeprilysines_ES
dc.subject.kobiosynthesises_ES
dc.subject.koNeprilysines_ES
dc.subject.kogeneticses_ES
dc.subject.koRatses_ES
dc.subject.koRats, Wistares_ES
dc.subject.koVentral Tegmental Areaes_ES
dc.subject.kodrug effectses_ES
dc.subject.koVentral Tegmental Areaes_ES
dc.subject.koenzymologyes_ES
dc.subject.koEthanoles_ES
dc.subject.koAntigens, CD13es_ES
dc.subject.koNeprilysines_ES
dc.titleActivity and expression of enkephalinase and aminopeptidase N in regions of the mesocorticolimbic system are selectively modified by acute ethanol administrationes_ES
dc.typearticlees_ES

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