Effects of melatonin on microtubule assembly depend on hormone concentration: role of melatonin as a calmodulin antagonist

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dc.contributor.affiliationInstituto Mexicano de Psiquiatría, Departamento de Neurofarmacología, México, D.F.es_ES
dc.creatorHuerto-Delgadillo, L.
dc.creatorAntón-Tay, F.
dc.creatorBenítez-King, G.
dc.creator.identificador"BEKG540727MDFNNL05">Benítez King, Gloria A.es_ES
dc.date.accessioned2017-06-29T04:17:41Z
dc.date.accessioned2026-03-27T14:35:12Z
dc.date.available2017-06-29T04:17:41Z
dc.date.issued1994es_ES
dc.date.published1994es_ES
dc.description.abstractotrodiomaMelatonin may play a key role in cytoskeletal rearrangements through its calmodulin antagonism. In the present work, we tested this hypothesis by studying melatonin effects on both microtubule polymerization in vitro and cytoskeletons in situ. Microtubule assembly is a dynamic process inhibited by Ca2+/calmodulin. Calmodulin antagonists prevent the inhibition by binding to Ca(2+)-activated calmodulin, thus causing microtubule enlargement. In the presence of calmodulin (5 microM) and CaCl2 (1 mM), polymerization at equilibrium was inhibited by 40%. Complete reversal of the Ca2+/calmodulin effect on microtubules was observed with 10(-9) M melatonin or with 10(-5) M trifluoperazine or 1 microgram/ml of compound 48/80. In the absence of Ca2+/calmodulin, melatonin at 10(-5) M inhibited tubulin polymerization like 10(-4) M trifluoperazine does. Melatonin effects on microtubule assembly at both nanomolar and micromolar ranges were corroborated in cytoskeletons in situ. Therefore, it is suggested that at a low concentration (10(-9) M), cytoskeletal melatonin effects are mediated by its antagonism to Ca2+/calmodulin. At a higher concentration (10(-5) M), non-specific binding of melatonin to tubulin occurs, thus overcoming the melatonin antagonism to Ca2+/calmodulin. The results support the hypothesis that under physiological conditions, melatonin synchronizes different body rhythms through cytoskeletal rearrangements mediated by its calmodulin antagonismes_ES
dc.description.monthSepes_ES
dc.identifier187es_ES
dc.identifier.citationJosé Artemio Hernández Martínezes_ES
dc.identifier.issn0742-3098es_ES
dc.identifier.numero2es_ES
dc.identifier.organizacionInstituto Mexicano de Psiquiatríaes_ES
dc.identifier.paginacion55-62es_ES
dc.identifier.placeDinamarcaes_ES
dc.identifier.urihttps://doi.org/10.1111/j.1600-079X.1994.tb00114.xes_ES
dc.identifier.urihttps://repositorio.inprf.gob.mx/handle/123456789/4881
dc.identifier.volumen17es_ES
dc.language.isoenges_ES
dc.relation17 (2) 55-62 p.es_ES
dc.relationversión del editores_ES
dc.relation.jnabreviadoJ PINEAL RESes_ES
dc.relation.journalJournal of Pineal Researches_ES
dc.rightsacceso cerradoes_ES
dc.titleEffects of melatonin on microtubule assembly depend on hormone concentration: role of melatonin as a calmodulin antagonistes_ES
dc.typearticlees_ES

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