Effect of A549 neuroendocrine differentiation on cytotoxic immune response

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dc.contributor.affiliationFacultad de QuímicaUniversidad Autónoma de Querétaro, Querétaro, Mexico
dc.contributor.emaillcbsq@yahoo.com (L. C. Berumen)
dc.creatorMendieta, Irasema
dc.creatorNuñez-Anita, Rosa Elvira
dc.creatorPérez-Sánchez, Gilberto
dc.creatorPavón, Lenin
dc.creatorRodríguez-Cruz, Alfredo
dc.creatorGarcía-Alcocer, Guadalupe
dc.creatorBerumen, Laura Cristina
dc.date2018
dc.date.accessioned2026-07-02T15:15:04Z
dc.date.issued2018
dc.date.published2018
dc.descriptionThe present study was designed to determine the effects of factors secreted by the lung adenocarcinoma cell line with the neuroendocrine phenotype, A549NED, on cytotoxic T lymphocytes (CTLs) activity in vitro A perspective that integrates the nervous, endocrine and immune system in cancer research is essential to understand the complexity of dynamic interactions in tumours. Extensive clinical research suggests that neuroendocrine differentiation (NED) is correlated with worse patient outcomes; however, little is known regarding the effects of neuroendocrine factors on the communication between the immune system and neoplastic cells. The human lung cancer cell line A549 was induced to NED (A549NED) using cAMP-elevating agents. The A549NED cells showed changes in cell morphology, an inhibition of proliferation, an overexpression of chromogranin and a differential pattern of biogenic amine production (decreased dopamine and increased serotonin [5-HT] levels). Using co-cultures to determine the cytolytic CTLs activity on target cells, we showed that the acquisition of NED inhibits the decrease in the viability of the target cells and release of fluorescence. Additionally, the conditioned medium of A549NED and 5-HT considerably decreased the viability and proliferation of the Jurkat cells after 24 h. Thus, our study successfully generated a neuroendocrine phenotype from the A549 cell line. In co-cultures with CTLs, the pattern of secretion by A549NED impaired the proliferation and cytotoxic activity of CTLs, which might be partly explained by the increased release of 5-HT.
dc.formatPDF
dc.identifierJC22NC18
dc.identifier.doi10.1530/EC-18-0145
dc.identifier.eissn2049-3614
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñiz
dc.identifier.placeInglaterra
dc.identifier.urihttps://repositorio.inprf.gob.mx/handle/123456789/178
dc.identifier.urihttps://doi.org/10.1530/EC-18-0145
dc.language.isoeng
dc.publisherBioScientifica
dc.relation7(5):791-802
dc.relation.jnabreviadoENDOCR CONNECT
dc.relation.journalEndocrine Connections
dc.rightsAcceso Cerrado
dc.subject.kwLung cancer
dc.subject.kwNeuroendocrine tumours
dc.subject.kwNeuroendocrine differentiation
dc.subject.kwTransdifferentiation
dc.subject.kwAntitumour response
dc.subject.kwImmunomodulator
dc.titleEffect of A549 neuroendocrine differentiation on cytotoxic immune response
dc.typeArtículo

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