Concomitant Treatment with Doxycycline and Rifampicin in Balb/c Mice Infected with Brucella abortus 2308 Fails to Reduce Inflammation and Motor Disability

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dc.contributor.affiliationLaboratorio de Inmunología Celular, Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City 11340, Mexico
dc.contributor.emaillkuriaki@inprf.gob.mx (L.P.); mlafont@ipn.mx (M.C.M.-L.)
dc.creatorMaldonado-García, José Luis
dc.creatorAlvarez-Herrera, Samantha
dc.creatorPérez-Sánchez, Gilberto
dc.creatorBecerril-Villanueva, Enrique
dc.creatorPavón, Lenin
dc.creatorTesoro-Cruz, Emiliano
dc.creatorGirón-Pérez, Manuel Iván
dc.creatorHurtado-Alvarado, Gabriela
dc.creatorDamián-Morales, Gabriela
dc.creatorLópez-Santiago, Rubén
dc.creatorMoreno-Lafont, Martha C.
dc.creator.identificadorhttps://orcid.org/0000-0003-2694-1290 (Maldonado-García, José Luis)
dc.creator.identificadorhttps://orcid.org/0000-0001-9747-885X (Alvarez-Herrera, Samantha)
dc.creator.identificadorhttps://orcid.org/0000-0003-3878-0631 (Pérez-Sánchez, Gilberto)
dc.creator.identificadorhttps://orcid.org/0000-0001-6808-6590 (Girón-Pérez, Manuel Iván)
dc.creator.identificadorhttps://orcid.org/0000-0003-3629-3813 (López-Santiago, Rubén)
dc.date2024
dc.date.accessioned2026-06-22T20:31:34Z
dc.date.issued2024
dc.date.published2024
dc.descriptionBrucellosis is an infection widely distributed around the world, and in some countries it is considered a public health problem. Brucellosis causes insidious symptoms that make it difficult to diagnose. Infection can also trigger chronic pain and neuropsychiatric complications. Antibiotics are not always effective to eradicate infection, contributing to chronicity. We aimed to investigate the effects of antibiotic treatment on proinflammatory cytokines, neurotransmitters, corticosterone, and behavior in a murine model of infecrion of B. abortus strain 2308. Four study groups were created: (a) control; (b) antibiotic control; (c) infected with B. abortus 2308; and (d) infected and treated with rifampicin and doxycycline. We determined B. abortus 2308 colony-forming units (CFUs), the count of dendritic cells, and macrophages in the spleen; serum levels of cytokines and corticosterone; levels of serotonin, dopamine, epinephrine, and norepinephrine in the brain; and equilibrium, physical strength, anxiety, and hopelessness tests. The infected and treated mice group was compared with the control and infected mice to assess whether treatment is sufficient to recover neuroimmunoendocrine parameters. Our results showed that despite the treatment of brucellosis with rifampicin and doxycycline, antibiotic-treated mice showed a persistence of B. abortus 2308 CFUs, an increased count in macrophage number, and higher circulating levels of corticosterone. Furthermore, the levels of IL-12, IL-6, and TNF-α remained higher. We found a decrease in muscular strength and equilibrium concomitant to changes in neurotransmitters in the hippocampus, cerebellum, and frontal cortex. Our data suggest that the remaining bacterial load after antibiotic administration favors inflammatory, neurochemical, and behavioral alterations, partly explaining the widespread and paradoxical symptomatology experienced by patients with chronic brucellosis.
dc.formatPDF
dc.identifierJC26NC24
dc.identifier.doi10.3390/ph17050638
dc.identifier.eissn1424-8247
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñiz
dc.identifier.placeSuiza
dc.identifier.urihttps://repositorio.inprf.gob.mx/handle/123456789/131
dc.identifier.urihttps://doi.org/10.3390/ph17050638
dc.language.isoeng
dc.publisherMDPI
dc.relation17(5):638
dc.relation.jnabreviadoPHARMACEUTICALS (BASEL)
dc.relation.journalPharmaceuticals
dc.rightsAcceso Cerrado
dc.subject.kwBrucellosis
dc.subject.kwDoxycycline
dc.subject.kwRifampicin
dc.subject.kwBehavioral test
dc.subject.kwInflammatory response
dc.subject.kwNeurotransmitters
dc.titleConcomitant Treatment with Doxycycline and Rifampicin in Balb/c Mice Infected with Brucella abortus 2308 Fails to Reduce Inflammation and Motor Disability
dc.typeArtículo

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