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Pharmacological evaluation of the anxiolytic-like effects of an aqueous extract of the Raphanus sativus L. sprouts in mice

dc.creatorHernández-Sánchez, Laura Yunuenes_ES
dc.creatorGonzález-Trujano, María Evaes_ES
dc.creatorMoreno, Diego A.es_ES
dc.creatorVibrans, Heikees_ES
dc.creatorCastillo-Juárez, Israeles_ES
dc.creatorDorazco-González, Alejandroes_ES
dc.creatorSoto-Hernández, Marcoses_ES
dc.date2023
dc.date.accessioned2025-03-14T18:18:47Z
dc.date.available2025-03-14T18:18:47Z
dc.date.issued2023
dc.identifierJC37es_ES
dc.identifier.issn0753-3322
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/8248
dc.identifier.urihttps://doi.org/10.1016/j.biopha.2023.114579
dc.descriptionRaphanus sativus L. (Brassicaceae), commonly known as radish, is consumed worldwide as a vegetable. However, its benefits on mental health are unknown. The aim of this study was to evaluate its anxiolytic-like effects and safety using different experimental models. An aqueous extract of R. sativus sprouts (AERSS) was pharmacologically evaluated by intraperitoneal route (i.p.) at 10, 30, and 100 mg/kg and orally (p.o.) at 500 mg/kg on behavior by using open-field and plus-maze tests. In addition, its acute toxicity (LD50) was determined by the Lorke's method. Diazepam (1 mg/kg, i.p.) and buspirone (4 mg/kg, i.p.) were the reference drugs. A significant and anxiolytic-like dosage of AERSS (30 mg/kg, i.p.) resembling the effects of reference drugs was chosen to explore the involvement of GABAA/BDZs site (flumazenil, 5 mg/kg, i.p.) and serotonin 5-HT1A receptors (WAY100635, 1 mg/kg, i.p.) as a possible mechanism of action. A 500 mg/kg, p.o. dosage of AERSS produced an anxiolytic-like response equivalent to 100 mg/kg, i.p. No acute toxicity was observed since a LD50 > 2000 mg/kg, i.p. The phytochemical analysis allowed the identification and quantification of major presence of sulforaphene (2500 µM), sulforaphane (15 µM), iberin (0.75 µM), and indol-3-carbinol (0.75 µM), as major constituents. Both the GABAA/BDZs site and serotonin 5-HT1A receptors were involved in the anxiolytic-like activity of AERSS, depending on the pharmacological parameter or the experimental assay tested. Our results demonstrate that the anxiolytic activity of R. sativus sprouts involves GABAA/BDZs site and serotonin 5-HT1A receptors supporting its health benefits in the treatment of anxiety beyond the satisfaction of basic nutritional needs.es_ES
dc.formatPDFes_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relation162:114579
dc.rightsAcceso Cerradoes_ES
dc.titlePharmacological evaluation of the anxiolytic-like effects of an aqueous extract of the Raphanus sativus L. sprouts in micees_ES
dc.typeArtículoes_ES
dc.contributor.affiliationPosgrado en Botánica, Colegio de Postgraduados Campus Montecillo, Carretera México-Texcoco Km. 36.5, Montecillo, Texcoco 56230, Estado de México, Mexico
dc.contributor.emaillauyunuen41@gmail.com (L.Y. Hernández-Sánchez), evag@imp.edu.mx, evagontru@yahoo.com.mx (M.E. González-Trujano), dmoreno@cebas.csic.es (D.A. Moreno), heike@colpos.mx (H. Vibrans), israel.castillo@colpos.mx (I. Castillo-Juárez), adg@unam.mx (A. Dorazco-González), msoto@colpos.mx (M. Soto-Hernández).
dc.relation.jnabreviadoBIOMED PHARMACOTHER
dc.relation.journalBiomedicine & Pharmacotherapy
dc.identifier.placeFrancia
dc.date.published2023
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñiz
dc.identifier.eissn1950-6007
dc.identifier.doi10.1016/j.biopha.2023.114579
dc.subject.kwAnxiety
dc.subject.kwBrassicaceae
dc.subject.kwGlucosinolates
dc.subject.kwMexican traditional medicine
dc.subject.kwSulforaphane


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