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Further evidence of the melatonin calmodulin interaction: effect on CaMKII activity

dc.creatorArgueta, Jesúses_ES
dc.creatorSolís-Chagoyán, Héctores_ES
dc.creatorEstrada-Reyes, Rosaes_ES
dc.creatorConstantino-Jonapa, Luis A.es_ES
dc.creatorOikawa-Sala, Juliánes_ES
dc.creatorVelázquez-Moctezuma, Javieres_ES
dc.creatorBenítez-King, Gloriaes_ES
dc.date2022
dc.date.accessioned2024-12-23T17:17:12Z
dc.date.available2024-12-23T17:17:12Z
dc.date.issued2022
dc.identifierOE18IC22es_ES
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/8182
dc.identifier.urihttps://doi.org/10.3390/ijms23052479
dc.descriptionMelatonin (MEL) is a pleiotropic indolamine that reaches multiple intracellular targets. Among these, MEL binds to calmodulin (CaM) with high affinity. In presence of Ca2+, CaM binds to CaM-dependent kinase II (CaMKII). The Ca2+-CaM/CaMKII pathway regulates a myriad of brain functions in different cellular compartments. Evidence showing the regulation of this cellular pathway by MEL is scarce. Thus, our main objective was to study the interaction of MEL with CaM and its effects on CaMKII activity in two microenvironments (aqueous and lipidic) naturally occurring within the cell. In addition, colocalization of MEL with CaM in vivo was explored in mice brain hippocampus. In vitro CaM-MEL interaction and the structural conformations of CaM in the presence of this indoleamine were assessed through electrophoretic mobility and isoelectric point. The functional consequence of this interaction was evaluated by measuring CaMKII activity. Ca2+-CaM-MEL increased the activity of CaMKII in aqueous buffer but reduced the kinase activity in lipid buffer. Importantly, MEL colocalizes in vivo with Ca2+-CaM in the hippocampus. Our evidence suggests that MEL regulates the key cellular Ca2+-CaM/CaMKII pathway and might explain why physiological MEL concentrations reduce CaMKII activity in some experimental conditions, while in others it drives biological processes through activation of this kinase.es_ES
dc.formatPDFes_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.relation23(5):2479
dc.rightsAcceso Cerradoes_ES
dc.titleFurther evidence of the melatonin calmodulin interaction: effect on CaMKII activityes_ES
dc.typeArtículoes_ES
dc.contributor.affiliationLaboratorio de Neurofarmacología, Subdirección de Investigaciones Clínicas, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, Calzada México-Xochimilco 101, San Lorenzo Huipulco, Tlalpan, Mexico City 14370, Mexico
dc.contributor.emailbekin@imp.edu.mx, bekin54@hotmail.com ( Gloria Benítez-King)
dc.relation.jnabreviadoINT J MOL SCI
dc.relation.journalInternational Journal of Molecular Sciences
dc.identifier.placeSuiza
dc.date.published2022
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñiz
dc.identifier.eissn1422-0067
dc.identifier.doi10.3390/ijms23052479
dc.subject.kwCalmodulin
dc.subject.kwMelatonin
dc.subject.kwCalmodulin kinase II
dc.subject.kwColocalization
dc.subject.kwHippocampus


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