Mostrar el registro sencillo del ítem

Origin of thyrotropin-releasing hormone neurons that innervate the tuberomammillary nuclei

dc.creatorSánchez-Jaramillo, Edithes_ES
dc.creatorWittmann, Gábores_ES
dc.creatorMenyhért, Judites_ES
dc.creatorSingru, Prafules_ES
dc.creatorGómez-González, Gabriela B.es_ES
dc.creatorSánchez-Islas, Eduardoes_ES
dc.creatorYáñez-Recendis, Nashielyes_ES
dc.creatorPimentel-Cabrera, Jaime Arturoes_ES
dc.creatorLeón-Olea, Marthaes_ES
dc.creatorGereben, Balázses_ES
dc.creatorFekete, Csabaes_ES
dc.creatorCharli, Jean-Louises_ES
dc.creatorLechan, Ronald M.es_ES
dc.date2022
dc.date.accessioned2024-12-18T20:51:14Z
dc.date.available2024-12-18T20:51:14Z
dc.date.issued2022
dc.identifierJC23NC22es_ES
dc.identifier.issn1863-2653
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/8167
dc.identifier.urihttps://doi.org/10.1007/s00429-022-02527-5
dc.descriptionHypophysiotropic thyrotropin-releasing hormone (TRH) neurons function as metabolic sensors that regulate the thyroid axis and energy homeostasis. Less is known about the role of other hypothalamic TRH neurons. As central administration of TRH decreases food intake and increases histamine in the tuberomammillary nuclei (TMN), and TMN histamine neurons are densely innervated by TRH fibers from an unknown origin, we mapped the location of TRH neurons that project to the TMN. The retrograde tracer, cholera toxin B subunit (CTB), was injected into the TMN E1-E2, E4-E5 subdivisions of adult Sprague-Dawley male rats. TMN projecting neurons were observed in the septum, preoptic area, bed nucleus of the stria terminalis (BNST), perifornical area, anterior paraventricular nucleus, peduncular and tuberal lateral hypothalamus (TuLH), suprachiasmatic nucleus and medial amygdala. However, CTB/pro-TRH178-199 double-labeled cells were only found in the TuLH. The specificity of the retrograde tract-tracing result was confirmed by administering the anterograde tracer, Phaseolus vulgaris leuco-agglutinin (PHAL) into the TuLH. Double-labeled PHAL-pro-TRH boutons were identified in all subdivisions of the TMN. TMN neurons double-labeled for histidine decarboxylase (Hdc)/PHAL, Hdc/Trh receptor (Trhr), and Hdc/Trh. Further confirmation of a TuLH-TRH neuronal projection to the TMN was established in a transgenic mouse that expresses Cre recombinase in TRH-producing cells following microinjection of a Cre recombinase-dependent AAV that expresses mCherry into the TuLH. We conclude that, in rodents, the TRH innervation of TMN originates in part from TRH neurons in the TuLH, and that this TRH population may contribute to regulate energy homeostasis through histamine Trhr-positive neurons of the TMN.es_ES
dc.formatPDFes_ES
dc.language.isoenges_ES
dc.publisherSpringeres_ES
dc.relation227(7):2329-2347
dc.rightsAcceso Cerradoes_ES
dc.titleOrigin of thyrotropin-releasing hormone neurons that innervate the tuberomammillary nucleies_ES
dc.typeArtículoes_ES
dc.contributor.affiliationLaboratorio de Neuroendocrinología Molecular, Dirección de Investigaciones en Neurociencias, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, 14370, México, CDMX, México
dc.contributor.emailesanchez@imp.edu.mx (Edith Sánchez-Jaramillo)
dc.relation.jnabreviadoBRAIN STRUCT FUNCT
dc.relation.journalBrain Structure & Function
dc.identifier.placeAlemania
dc.date.published2022
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñiz
dc.identifier.eissn1863-2661
dc.identifier.doi10.1007/s00429-022-02527-5
dc.subject.kwTRH
dc.subject.kwHistamine
dc.subject.kwRetrograde
dc.subject.kwAnterograde
dc.subject.kwLateral hypothalamus
dc.subject.kwTrh–Cre transgene


Ficheros en el ítem

FicherosTamañoFormatoVer

No hay ficheros asociados a este ítem.

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem