Mostrar el registro sencillo del ítem

Specific localization of an auto-inhibition mechanism at presynaptic terminals of identified serotonergic neurons

dc.creatorGarcía-Ávila, Miriames_ES
dc.creatorTorres, Ximenaes_ES
dc.creatorCercós, Montserrat G.es_ES
dc.creatorTrueta, Citlalies_ES
dc.date2021
dc.date.accessioned2024-06-21T21:10:41Z
dc.date.available2024-06-21T21:10:41Z
dc.date.issued2021
dc.identifierJC34NC21es_ES
dc.identifier.issn0306-4522
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/8015
dc.identifier.urihttps://doi.org/10.1016/j.neuroscience.2020.12.015
dc.descriptionAuto-regulation mechanisms in serotonergic neurons regulate their electrical activity and secretion. Since these neurons release serotonin from different structural compartments - including presynaptic terminals, soma, axons and dendrites - through different mechanisms, autoregulation mechanisms are also likely to be different at each compartment. Here we show that a chloride-mediated auto-inhibitory mechanism is exclusively localized at presynaptic terminals, but not at extrasynaptic release sites, in serotonergic Retzius neurons of the leech. An auto-inhibition response was observed immediately after intracellular stimulation with an electrode placed in the soma, in neurons that were isolated and cultured retaining an axonal stump, where presynaptic terminals are formed near the soma, but not in somata isolated without axon, where no synaptic terminals are formed, nor in neurons in the nerve ganglion, where terminals are electrotonically distant from the soma. Furthermore, no auto-inhibition response was detected in either condition during the longer time course of somatic secretion. This shows that the auto-inhibition effects are unique to nerve terminals. We further determined that serotonin released from peri-synaptic dense-core vesicles contributes to auto-inhibition in the terminals, since blockade of L-type calcium channels, which are required to stimulate extrasynaptic but not synaptic release, decreased the amplitude of the auto-inhibition response. Our results show that the auto-regulation mechanism at presynaptic terminals is unique and different from that described in the soma of these neurons, further highlighting the differences in the mechanisms regulating serotonin release from different neuronal compartments, which expand the possibilities of a single neuron to perform multiple functions in the nervous system.es_ES
dc.formatPDFes_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relation458:120-132
dc.rightsAcceso Cerradoes_ES
dc.titleSpecific localization of an auto-inhibition mechanism at presynaptic terminals of identified serotonergic neuronses_ES
dc.typeArtículoes_ES
dc.contributor.affiliationDepartamento de Neurofisiología, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, Calzada México-Xochimilco 101, San Lorenzo Huipulco, Tlalpan 14370, Ciudad de México, Mexico
dc.contributor.emailgmiriam@ciencias.unam.mx (M. García-Ávila) vila), ximena_torres@ciencias.unam.mx (X. Torres), montse@imp.edu.mx (M. G. Cercós), ctrueta@imp.edu.mx (C. Trueta)
dc.relation.jnabreviadoNEUROSCIENCE
dc.relation.journalNeuroscience
dc.identifier.placeEstados Unidos
dc.date.published2021
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñiz
dc.identifier.eissn1873-7544
dc.identifier.doi10.1016/j.neuroscience.2020.12.015
dc.subject.kwSerotonin
dc.subject.kwAuto-inhibition
dc.subject.kwCompartmentalization
dc.subject.kwPresynaptic terminal
dc.subject.kwPeri-synaptic release
dc.subject.kwLeech


Ficheros en el ítem

FicherosTamañoFormatoVer

No hay ficheros asociados a este ítem.

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem