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Interaction of NHE1 and TRPA1 activity in DRG neurons isolated from adult rats and its role in inflammatory nociception

dc.creatorMartínez-Rojas, Vladimir A.es_ES
dc.creatorSalinas-Abarca, Ana B.es_ES
dc.creatorGómez-Víquez, Norma L.es_ES
dc.creatorGranados-Soto, Vinicioes_ES
dc.creatorMercado, Franciscoes_ES
dc.creatorMurbartián, Janetes_ES
dc.date2021
dc.date.accessioned2024-06-03T19:08:54Z
dc.date.available2024-06-03T19:08:54Z
dc.date.issued2021
dc.identifierJC18NC21es_ES
dc.identifier.issn0306-4522
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/7991
dc.identifier.urihttps://doi.org/10.1016/j.neuroscience.2021.04.025
dc.descriptionTransient receptor potential ankyrin 1 (TRPA1) channel is expressed in a subset of nociceptive neurons. This channel integrates several nociceptive signals. Particularly, it is modulated by intracellular pH (pHi). Na+/H+ exchanger 1 (NHE1) contributes to the maintenance of pHi in nociceptors. However, it is currently unknown whether the interaction between TRPA1 and NHE1 contributes to the nociceptive processing. Thus, the purpose of this study was to assess the functional interaction between NHE1 and TRPA1 in small dorsal root ganglion (DRG) neurons from primary culture obtained from adult rats. Moreover, we also evaluated their possible interaction in acute and inflammatory pain. Zoniporide (selective NHE1 inhibitor) reduced pHi and increased intracellular calcium in a concentration-dependent fashion in DRG neurons. Zoniporide and allyl isothiocyanate (AITC, TRPA1 agonist) increased calcium transients in the same DRG neuron, whereas that A-967079 (TRPA1 antagonist) prevented the effect of zoniporide in DRG neurons. Repeated AITC induced TRPA1 desensitization and this effect was prevented by zoniporide. Both NHE1 and TRPA1 were localized at the membrane surface of DRG neurons in culture. Local peripheral zoniporide enhanced AITC-induced pronociception and this effect was prevented by A-967079. Likewise, zoniporide potentiated Complete Freund's Adjuvant (CFA)-induced hypersensitivity, effect which was prevented by A-967079 in vivo. CFA paw injection increased TRPA1 and decresed NHE1 protein expression in DRG. These results suggest a functional interaction between NHE1 and TRPA1 in DRG neurons in vitro. Moreover, data suggest that this interaction participates in acute and inflamatory pain conditions in vivo.es_ES
dc.formatPDFes_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relation465:154-165
dc.rightsAcceso Cerradoes_ES
dc.titleInteraction of NHE1 and TRPA1 activity in DRG neurons isolated from adult rats and its role in inflammatory nociceptiones_ES
dc.typeArtículoes_ES
dc.contributor.affiliationDepartamento de Farmacobiología, Cinvestav, South Campus, Mexico City, Mexico
dc.contributor.emailjmurbartian@gmail.com (J. Murbartián)
dc.relation.jnabreviadoNEUROSCIENCE
dc.relation.journalNeuroscience
dc.identifier.placeEstados Unidos
dc.date.published2021
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñiz
dc.identifier.eissn1873-7544
dc.identifier.doi10.1016/j.neuroscience.2021.04.025
dc.subject.kwIntracellular acidification
dc.subject.kwIntracellular calcium
dc.subject.kwInflammatory pain
dc.subject.kwNHE1
dc.subject.kwTRPA1


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