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Modulation of intracellular calcium concentration by D2-like DA receptor agonists in non-peptidergic DRG neurons is mediated mainly by D4 receptor activation

dc.creatorSegura-Chama, Pedroes_ES
dc.creatorLuis, Enoches_ES
dc.creatorAlmanza, Angélicaes_ES
dc.creatorPellicer, Franciscoes_ES
dc.creatorHernández-Cruz, Arturoes_ES
dc.creatorMercado, Franciscoes_ES
dc.date2020
dc.date.accessioned2023-06-12T18:11:37Z
dc.date.available2023-06-12T18:11:37Z
dc.date.issued2020
dc.identifierJC22NC20es_ES
dc.identifier.issn0304-3940
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/7710
dc.identifier.urihttps://doi.org/10.1016/j.neulet.2020.135267
dc.descriptionNociceptive stimuli attributes are codified in the periphery; at this level, D2-like dopamine (DA) receptor activation decreases the high voltage-gated Ca2+ current predominantly in mechanonociceptive neurons, which explains the presynaptic action mechanism of the antinociception produced by quinpirole when it is intrathecally administered in rats. However, the identity of D2-like DA receptor subtype that mediates this effect remains unknown. To answer this question, we used Fluo-4-based Ca2+ microfluorometry to study the depolarization-elicited [Ca2+]i increase in small non-peptidergic DRG neurons (identified by its binding to the Isolectin B4), and to test the effect of D2-like DA receptor activation by quinpirole in presence of selective antagonists for D2, D3, and D4 DA receptors. The results showed a significantly greater contribution of the D4 DA receptor in the down-modulation of depolarization-elicited [Ca2+]i increase in small non-peptidergic DRG neurons compared to the other receptors. Although the D2 and D3 receptor antagonists also slightly inhibited the effect of quinpirole, their effects were significantly weaker than those of the D4 receptor antagonist. Furthermore, we showed that quinpirole selectively inhibits the CaV2.2 Ca2+ channels. Our results suggest that the activation of the D4 DA receptors is a promising strategy for pain management at the spinal cord level.es_ES
dc.formatPDFes_ES
dc.language.isoenges_ES
dc.publisherElsevier Scientific Publishers Irelandes_ES
dc.relation736:135267
dc.rightsAcceso Cerradoes_ES
dc.titleModulation of intracellular calcium concentration by D2-like DA receptor agonists in non-peptidergic DRG neurons is mediated mainly by D4 receptor activationes_ES
dc.typeArtículoes_ES
dc.contributor.affiliationLaboratorio de Fisiología Celular, Dirección de Investigaciones en Neurociencias, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, Ciudad de México, Mexico
dc.contributor.emailfmercado@imp.edu.mx (F. Mercado)
dc.relation.jnabreviadoNEUROSCI LETT
dc.relation.journalNeuroscience Letters
dc.identifier.placeIrlanda
dc.date.published2020
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñiz
dc.identifier.eissn1872-7972
dc.identifier.doi10.1016/j.neulet.2020.135267
dc.subject.kwD2-like receptors
dc.subject.kwD4 receptor
dc.subject.kwDepolarization-elicited [Ca 2+]i increase
dc.subject.kwCa 2+ microfluorometry
dc.subject.kwSmall non-peptidergic DRG neurons
dc.subject.kwCa V2.2 Ca 2+ channels


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