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Intra-accumbal orexin-1 receptor inhibition prevents the anxiolytic-like effect of ethanol and leads to increases in orexin-A content and receptor expression
| dc.creator | Morales-Mulia Marcela | |
| dc.date | 2019 | |
| dc.date.accessioned | 2023-01-17T18:24:30Z | |
| dc.date.available | 2023-01-17T18:24:30Z | |
| dc.date.issued | 2019 | |
| dc.identifier.issn | 0091-3057 | |
| dc.identifier.uri | http://repositorio.inprf.gob.mx/handle/123456789/7666 | |
| dc.identifier.uri | https://doi.org/10.1016/j.pbb.2019.172761 | |
| dc.description | Alcohol use is frequently associated with mood disorders. Similarly, individuals suffering from these disorders have a higher risk of developing alcoholism. Several reports have implicated orexin signaling in different be haviors related to alcohol consumption, whereas antagonists block these actions. However, the involvement of orexin-1-receptor (Orx1R) in ethanol-induced anxiolysis remains relatively unexplored. The purpose of this study was to investigate whether intra-accumbal inhibition of Orx1R blocks the anxiolytic-like effect of ethanol and to determine if ethanol administration modifies orexin-A content and Orx1R expression in the nucleus accumbens (NAc). The elevated-plus-maze test (EPM-test) was used to measure anxiety; orexin-A content and Orx1R ex pression were determined by enzyme-immunoassay and western blot, respectively. The results showed that the pretreatment with a selective antagonist of Orx1R, SB-334867 (SB, 3 μg/side), prevents the anxiolytic-like be havior induced by acute ethanol (2.5 g/kg). SB-334867 per se had no effect on anxiety levels. Pretreatment with SB-334867 followed by ethanol (SB + Et) increased orexin-A content and Orx1R levels in the NAc in comparison to the groups that only received ethanol (V + Et) or SB-334867 (SB + S). Ethanol treatment significantly aug mented Orx1R expression but not the peptide content. The increase in orexin-A observed in SB + Et animals could be due in part to the inhibition of Orx1R, since SB-334867 prevents the binding of orexin-A to the receptor. This increase in orexin-A may, in turn, induce an up-regulation of receptor. Other possible explanations were discussed. In general, these findings suggest that orexin-A contributes largely to expression of ethanol-induced anxiolytic-like effect through the signaling of Orx1R in the NAc. | |
| dc.format | ||
| dc.language.iso | eng | |
| dc.publisher | Elsevier | |
| dc.relation | 185:172761 | |
| dc.rights | Acceso Cerrado | |
| dc.title | Intra-accumbal orexin-1 receptor inhibition prevents the anxiolytic-like effect of ethanol and leads to increases in orexin-A content and receptor expression | es_ES |
| dc.type | Artículo | |
| dc.contributor.affiliation | Bases Moleculares de las Adicciones, Subdirección de Investigaciones Clínicas, INPRFM, México D.F., Mexico | |
| dc.contributor.email | mmulia@imp.edu.mx (Marcela Morales-Mulia) | |
| dc.relation.jnabreviado | PHARMACOL BIOCHEM BEHAV | |
| dc.relation.journal | Pharmacology, Biochemistry and Behavior | |
| dc.identifier.place | Estados Unidos | |
| dc.date.published | 2019 | |
| dc.identifier.organizacion | Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz | |
| dc.identifier.eissn | 1873-5177 | |
| dc.identifier.doi | 10.1016/j.pbb.2019.172761 | |
| dc.subject.kw | Orexin-A | |
| dc.subject.kw | Orexin-1-receptors | |
| dc.subject.kw | Ethanol | |
| dc.subject.kw | Anxiety | |
| dc.subject.kw | SB-334867 | |
| dc.subject.kw | Nucleus accumbens |
