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Maternal manipulation during late gestation (GDs 17-20) enhances ethanol consumption and promotes changes and opioid mRNA expression in infant rats

dc.creatorGuttlein, Larisaes_ES
dc.creatorMacchione, Ana Fabiolaes_ES
dc.creatorHernández-Fonseca, Karlaes_ES
dc.creatorHaymal, Olga Beatrizes_ES
dc.creatorMolina, Juan Carloses_ES
dc.creatorMéndez Ubach, Milagroses_ES
dc.creatorAbate, Paulaes_ES
dc.date2019
dc.date.accessioned2022-11-29T17:48:39Z
dc.date.available2022-11-29T17:48:39Z
dc.date.issued2019
dc.identifierMT08IC22
dc.identifier.issn0166-4328
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/7642
dc.identifier.urihttps://doi.org/10.1016/j.bbr.2019.111908
dc.descriptionFetal ethanol experience generates learning and memories capable to increase ethanol consummatory behaviors during infancy. Opioid system seems to be involved in mediating those alcohol-related behaviors. In this work, we proposed to study the impact of prenatal exposure to a moderate ethanol dose, upon ingestion of the drug and possible ethanol-induced molecular changes on opioid precursor peptides (POMC, Pro-enk and Pro-DYN) and receptors (MOR, DOR and KOR) mRNA expression, in hypothalamus. Pregnant rats received during gestational days (GDs) 17-20, a daily intragastric (i.g.) administration with 2g/kg ethanol or water. A third group of dams was left undisturbed during pregnancy (Unmanipulated group). Intake test was conducted at postnatal days (PDs) 14-15. Three groups of pups were performed: control (no intake test), water (vehicle) and 5% ethanol. At the end of intake test blood samples were taken to quantify blood ethanol concentrations (BECs) and hy pothalamus sections were obtained to perform qRT-PRC assessment of opioid precursor peptides and receptors. The analysis of the consummatory responses (% of consumption) and pharmacokinetic profiles (BECs) suggested that maternal manipulation induced by i.g. intubations, during the last four days of gestation (whenever ethanol or water), are sufficient to induce infantile ethanol intake during infancy. Gene expression from the hypotha lamus of unmanipulated group revealed that infantile ingestive experiences with ethanol can down-regulate expression of mRNA Pro-Dyn and up-regulate mRNA expression of MOR and KOR. Finally, MOR mRNA ex pression was attenuated by prenatal i.g. manipulation in pups exposed to 5% ethanol.
dc.formatPDF
dc.language.isoeng
dc.publisherElsevier
dc.titleMaternal manipulation during late gestation (GDs 17-20) enhances ethanol consumption and promotes changes and opioid mRNA expression in infant ratses_ES
dc.contributor.affiliationInstituto de Investigación Médica M. y M. Ferreyra, INIMEC-CONICET-Universidad Nacional de Córdoba, Córdoba, C.P. 5016, Argentina
dc.contributor.emailubach@imp.edu.mx (Méndez Ubach, M.) pabate@unc.edu.ar (Abate, P.)
dc.relation.jnabreviadoBEHAV BRAIN RES
dc.identifier.placePaíses bajos
dc.date.published2019
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñiz
dc.identifier.eissn0166-4328
dc.identifier.doi10.1016/j.bbr.2019.111908


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