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Cannabis users show enhanced expression of CB1-5HT2A receptor heteromers in olfactory neuroepithelium cells

dc.creatorGalindo, Lilianaes_ES
dc.creatorMoreno. Estefaníaes_ES
dc.creatorLópez-Armenta, Fernandoes_ES
dc.creatorGuinart, Danieles_ES
dc.creatorCuenca-Royo, Aidaes_ES
dc.creatorIzquierdo-Serra, Mercèes_ES
dc.creatorXicota, Lauraes_ES
dc.creatorFernandez, Cristinaes_ES
dc.creatorMenoyo, Estheres_ES
dc.creatorFernández-Fernández, José M.es_ES
dc.creatorBenítez-King, Gloriaes_ES
dc.creatorCanela, Enric I.es_ES
dc.creatorCasadó, Vicentes_ES
dc.creatorPérez, Víctores_ES
dc.creatorde la Torre, Rafaeles_ES
dc.creatorRobledo, Patriciaes_ES
dc.date2018
dc.date.accessioned2022-11-29T16:22:25Z
dc.date.available2022-11-29T16:22:25Z
dc.date.issued2018
dc.identifierMT06IC22
dc.identifier.issn0893-7648
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/7640
dc.identifier.urihttps://doi.org/10.1007/s12035-017-0833-7
dc.descriptionCannabinoid CB1 receptors (CB1R) and serotonergic 2A receptors (5HT2AR) form heteromers in the brain of mice where they mediate the cognitive deficits produced by delta-9-tetrahydrocannabinol. However, it is still unknown whether the expression of this hetero dimer is modulated by chronic cannabis use in humans. In this study, we investigated the expression levels and functionality of CB1R 5HT2AR heteromers in human olfactory neuroepithelium (ON) cells of cannabis users and control subjects, and determined their molecular characteristics through adenylate cyclase and the ERK 1/2 pathway signaling studies. We also assessed whether heteromer expression levels correlated with cannabis consumption and cognitive performance in neuropsychological tests. ON cells from controls and cannabis users expressed neuronal markers such as βIII-tubulin and nestin, displayed similar expression levels of genes related to cellular self-renewal, stem cell differentiation, and generation of neural crest cells, and showed comparable Na+ currents in patch clamp recordings. Interestingly, CB1R-5HT2AR heteromer expression was significantly increased in cannabis users and positively correlated with the amount of cannabis consumed, and negatively with age of onset of cannabis use. In addition, a negative correlation was found between heteromer expression levels and attention and working memory performance in cannabis users and control subjects. Our findings suggest that cannabis consumption regulates the formation of CB1R-5HT2AR heteromers, and may have a key role in cognitive processing. These heterodimers could be potential new targets to develop treatment alternatives for cognitive impairments.
dc.formatPDF
dc.language.isoeng
dc.publisherHumana Press
dc.relation55(8)6347–6361
dc.rightsAcceso Cerrado
dc.titleCannabis users show enhanced expression of CB1-5HT2A receptor heteromers in olfactory neuroepithelium cellses_ES
dc.typeArtículo
dc.contributor.affiliationNeuropsychiatry and Addictions Institute (INAD) of Parc de Salut Mar, Centro de Investigación Biomédica En Red de Salud Mental G21, Mental Health Research Group, IMIM-Hospital del Mar Research Institute, Barcelona, Spain
dc.contributor.emailprobledo@imim.es (Robledo, Patricia)
dc.relation.jnabreviadoMOL NEUROBIOL
dc.relation.journalMolecular Neurobiology
dc.identifier.placeEstados Unidos
dc.date.published2018
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñiz
dc.identifier.eissn1559-1182
dc.identifier.doi10.1007/s12035-017-0833-7
dc.subject.kwCB1R-5HT2AR Heteromers
dc.subject.kwCannabis
dc.subject.kwCognitive
dc.subject.kwProgenitor Cells
dc.subject.kwHuman Olfactory Neuroepithelium


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