Mostrar el registro sencillo del ítem

Purinergic signaling pathway in human olfactory neuronal precursor cells

dc.creatorSolís-Chagoyán, Héctor Solíses_ES
dc.creatorFlores-Soto, Edgar Floreses_ES
dc.creatorValdés-Tovar, Marcelaes_ES
dc.creatorCercós, Montserrat G.es_ES
dc.creatorCalixto, Eduardoes_ES
dc.creatorMontaño, Luis M.es_ES
dc.creatorBarajas-López, Carloses_ES
dc.creatorSommer, Bettinaes_ES
dc.creatorAquino-Gálvez, Arnoldoes_ES
dc.creatorTrueta, Citlalies_ES
dc.creatorBenítez-King, Gloria A.es_ES
dc.date2019
dc.date.accessioned2022-11-15T19:49:02Z
dc.date.available2022-11-15T19:49:02Z
dc.date.issued2019
dc.identifierJC10NC22es_ES
dc.identifier.issn1687-966X
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/7623
dc.identifier.urihttps://doi.org/10.1155/2019/2728786
dc.descriptionExtracellular ATP and trophic factors released by exocytosis modulate in vivo proliferation, migration, and differentiation in multipotent stem cells (MpSC); however, the purinoceptors mediating this signaling remain uncharacterized in stem cells derived from the human olfactory epithelium (hOE). Our aim was to determine the purinergic pathway in isolated human olfactory neuronal precursor cells (hONPC) that exhibit MpSC features. Cloning by limiting dilution from a hOE heterogeneous primary culture was performed to obtain a culture predominantly constituted by hONPC. Effectiveness of cloning to isolate MpSC-like precursors was corroborated through immunodetection of specific protein markers and by functional criteria such as self-renewal, proliferation capability, and excitability of differentiated progeny. P2 receptor expression in hONPC was determined by Western blot, and the role of these purinoceptors in the ATP-induced exocytosis and changes in cytosolic Ca2+ ([Ca2+]i) were evaluated using the fluorescent indicators FM1-43 and Fura-2 AM, respectively. The clonal culture was enriched with SOX2 and OCT3/4 transcription factors; additionally, the proportion of nestin-immunopositive cells, the proliferation capability, and functionality of differentiated progeny remained unaltered through the long-term clonal culture. hONPC expressed P2X receptor subtypes 1, 3-5, and 7, as well as P2Y2, 4, 6, and 11; ATP induced both exocytosis and a transient [Ca2+]i increase predominantly by activation of metabotropic P2Y receptors. Results demonstrated for the first time that ex vivo-expressed functional P2 receptors in MpSC-like hONPC regulate exocytosis and Ca2+ signaling. This purinergic-triggered release of biochemical messengers to the extracellular milieu might be involved in the paracrine signaling among hOE cells.es_ES
dc.formatPDFes_ES
dc.language.isoenges_ES
dc.publisherHindawi Publishing Corporationes_ES
dc.relation2019: 2728786
dc.rightsAcceso Cerradoes_ES
dc.titlePurinergic signaling pathway in human olfactory neuronal precursor cellses_ES
dc.typeArtículoes_ES
dc.contributor.affiliationInstituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, Laboratorio de Neurofarmacología, Calzada México-Xochimilco 101, San Lorenzo Huipulco, CP 14370 Ciudad de México, Mexico
dc.contributor.emailhecsolch@imp.edu.mx (Héctor Solís-Chagoyán), bekin@imp.edu.mx (Gloria A. Benítez-King)
dc.relation.jnabreviadoSTEM CELLS INT
dc.relation.journalStem Cells International
dc.identifier.placeEstados Unidos
dc.date.published2019
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñiz
dc.identifier.eissn1687-9678
dc.identifier.doi10.1155/2019/2728786


Ficheros en el ítem

FicherosTamañoFormatoVer

No hay ficheros asociados a este ítem.

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem