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The nociceptin/orphanin FQ-like opioid peptide in nervous periesophageal ganglia of land snail Helix aspersa

dc.creatorLeón-Olea, Martha
dc.creatorMiller-Pérez, Carolina
dc.creatorSánchez-Islas, Eduardo
dc.creatorMendoza-Sotelo, José
dc.creatorGarduño-Gutiérrez, René
dc.creatorDe Gortari, Patricia
dc.creatorAmaya, María Isabel
dc.date.accessioned2017-06-30T04:04:47Z
dc.date.available2017-06-30T04:04:47Z
dc.date.issued2013es_ES
dc.identifier2492es_ES
dc.identifier.issn0006-8993es_ES
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/7133
dc.identifier.urihttps://doi.org/10.1016/j.brainres.2013.02.007es_ES
dc.language.isoenges_ES
dc.publisherELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSes_ES
dc.relation1505, 22-46 p.es_ES
dc.relationversión del editores_ES
dc.rightsacceso cerradoes_ES
dc.titleThe nociceptin/orphanin FQ-like opioid peptide in nervous periesophageal ganglia of land snail Helix aspersaes_ES
dc.typearticlees_ES
dc.contributor.affiliationInst Nacl Psiquiatria Ramon de la Fuente Muniz, Direcc Invest Neurociencias, Dept Neuromorfol Func, Av Mexico Xochimilco 101, Mexico City 14370, DF, Mexico.es_ES
dc.relation.jnabreviadoBRAIN RESes_ES
dc.relation.journalBrain researches_ES
dc.identifier.placeAmsterdames_ES
dc.date.published2013es_ES
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, México.es_ES
dc.identifier.eissn1872-6240es_ES
dc.identifier.doi10.1016/j.brainres.2013.02.007es_ES
dc.description.monthAbres_ES
dc.description.abstractotrodiomaThe neuropeptide nociceptin/orphanin FQ (N/OFQ) and its receptor are members of the endogenous opioid peptide family. In mammals N/OFQ modulates a variety of biological functions such as nociception, food intake, endocrine, control of neurotransmitter release, among others. In the molluscs Cepea nemoralis and Helix aspersa the administration of N/OFQ produces a thermopronociceptive effect. However, little is known about its existence and anatomic distribution in invertebrates. The aim of this study was to provide a detailed anatomical distribution of N/OFQ like peptide immunoreactivity (N/OFQ-IL), to quantify the tissue content of this peptide, as well as to demostrate molecular evidence of N/OFQ mRNA in the nervous tissue of periesophageal ganglia of the land snail H. aspersa. Immunohistochemical, immunocytochemical, radioimmunoanalysis (RIA) and reverse transcription-polymerase chain reaction (RT-PCR) techniques were used. With regard to RT-PCR, the primers to detect expression of mRNA transcripts from H. aspersa were derived from the rat N/OFQ opioid peptide. We show a wide distribution of N/OFQ-IL in neurons and fibers in all perioesophageal ganglia, fibers of the neuropile, nerves, periganglionar connective tissue, aortic wall and neurohemal sinuses. The total amount of N/OFQ-IL in the perioesophageal ganglia (7.75 +/- 1.75 pmol/g of tissue) quantified by RIA was similar to that found in mouse hypothalamus (10.1 +/- 1.6 pmol/g of tissue). In this study, we present molecular evidence of N/OFQ mRNA expression. Some N/OFQ-IL neurons have been identified as neuroendocrine or involved in olfaction, hydro-electrolyte regulation, feeding, and thermonociception. Therefore, we suggest that N/OFQ may participate in these snail functions. (C) 2013 Elsevier B.V. All rights reserved.es_ES


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