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MT2-like melatonin receptor modulates amplitude receptor potential in visual cells of crayfish during a 24-hour cycle

dc.creatorMendoza-Vargas, Leonor
dc.creatorSolís-Chagoyán, Héctor
dc.creatorBenítez-King, Gloria
dc.creatorFuentes-Pardo, Beatriz
dc.date.accessioned2017-06-30T04:00:13Z
dc.date.available2017-06-30T04:00:13Z
dc.date.issued2009es_ES
dc.identifier2443es_ES
dc.identifier.issn1095-6433es_ES
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/7084
dc.identifier.urihttps://doi.org/10.1016/j.cbpa.2009.07.025es_ES
dc.language.isoenges_ES
dc.publisherElsevier Science INC, 360 Park Ave South, New York, NY 10010-1710 USAes_ES
dc.relation154 (4) 486-492 p.es_ES
dc.relationversión del editores_ES
dc.rightsacceso cerradoes_ES
dc.titleMT2-like melatonin receptor modulates amplitude receptor potential in visual cells of crayfish during a 24-hour cyclees_ES
dc.typearticlees_ES
dc.contributor.affiliationUniv Nacl Autonoma Mexico, Dept Fisiol, Fac Med, Mexico City 04510, DF, Mexicoes_ES
dc.contributor.emailbfpardo@servidor.unam.mxes_ES
dc.relation.jnabreviadoCOMP BIOCHEM PHYSIOL A MOL INTEGR PHYSIOLes_ES
dc.relation.journalComparative biochemistry and physiology. Part A, Molecular & integrative physiologyes_ES
dc.identifier.placeNew Yorkes_ES
dc.date.published2009es_ES
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, México.es_ES
dc.identifier.eissn1531-4332es_ES
dc.identifier.doi10.1016/j.cbpa.2009.07.025es_ES
dc.description.monthDices_ES
dc.description.abstractotrodiomaRetinular photoreceptors are structures involved in the expression and synchronization of the circadian rhythm of sensitivity to light in crayfish. To determine whether melatonin possesses a differential effect upon the receptor potential (RP) amplitude of retinular photoreceptors circadian time (CT)-dependent, we conducted experiments by means of applying melatonin every 2 h during a 24-hour cycle. Melatonin with 100 nM increased RP amplitude during subjective day to a greater degree than during subjective night To determine whether MT2 melatonin receptors regulate the melatonin-produced effect, we carried out two experiments, circadian times (Crs) 6 and 18, which included the following: (1) application of the MT2 receptor selective agonist 8-M-PDOT and antagonist DH97, and (2) the specific binding of [I-125]-melatonin in eyestalk membranes. The amount of 10 nM of 8-M-PDOT increased RP amplitude in a similar manner to melatonin, and 1 nM DH97 abolished the increase produced by melatonin and 8-M-PDOT. Binding of [I-125]-melatonin was saturable and specific. Scatchard analysis revealed an affinity constant (K-d) of 1.1 nM and a total number of binding sites (B-max) of 6 fmol/mg protein at CT 6, and a K-d of 1.46 nM and B-max of 7 fmol/mg protein at CT 18. Our results indicate that melatonin increased RP amplitude of crayfish retinular photoreceptors through MT2-like melatonin receptors. These data support the idea that melatonin is a signal of darkness for the circadian system in crayfish retinular cells.es_ES
dc.subject.kwMelatonina-receptor MT2 ritmo circadianoes_ES
dc.subject.kwPotencial receptores_ES
dc.subject.kwFotorreceptoreses_ES
dc.subject.kwCangrejo de ríoes_ES
dc.subject.koMT2 melatonin-receptor circadian rhythmes_ES
dc.subject.koReceptor potentiales_ES
dc.subject.koPhotoreceptorses_ES
dc.subject.koCrayfishes_ES


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