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Methodologic pitfalls in measurement of 5-hydroxytriptamine uptake transporters in human platelets by [H-3]-paroxetine binding assay

dc.creatorJurado, N.
dc.creatorTorner, C.
dc.creatorHeinze, G.
dc.creatorLópez, G.
dc.creatorMendoza-Sotelo, J.
dc.creatorLazo-Langner, A.
dc.creatorMoreno, J.
dc.date.accessioned2017-06-30T03:59:37Z
dc.date.available2017-06-30T03:59:37Z
dc.date.issued2003es_ES
dc.identifier2436es_ES
dc.identifier.issn0188-4409es_ES
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/7077
dc.identifier.urihttps://doi.org/10.1016/j.arcmed.2003.03.001es_ES
dc.language.isoenges_ES
dc.publisherELSEVIER SCIENCE INC, 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USAes_ES
dc.relation34 (5) 422-427 p.es_ES
dc.relationversión del editores_ES
dc.rightsacceso cerradoes_ES
dc.titleMethodologic pitfalls in measurement of 5-hydroxytriptamine uptake transporters in human platelets by [H-3]-paroxetine binding assayes_ES
dc.typearticlees_ES
dc.contributor.affiliationInst Nacl Psiquiat Dr Ramon de la Fuente, Div Serv Clin, Lab Clin, Calzada Mexico Xochimilco 101,Col San Lorenzo Hui, Mexico City 14370, DF, Mexico.es_ES
dc.relation.jnabreviadoARCH MED RESes_ES
dc.relation.journalArchives of medical researches_ES
dc.identifier.placeNew Yorkes_ES
dc.date.published2003es_ES
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, México.es_ES
dc.identifier.eissn1873-5487es_ES
dc.identifier.doi10.1016/j.arcmed.2003.03.001es_ES
dc.description.monthSepes_ES
dc.description.abstractotrodiomaBackground. Studies in platelet of 5-HT uptake transporters have been performed using binding assay methodology designed for ligand-receptor interactions| however, uptake 4 transporters present requirements that may question the validity of these particular binding assays. Methods. To explore methodologic aspects that may be crucial to the validity of these assays, we studied the binding of [H-3]-paroxetine to platelet membranes of healthy subjects under different conditions of time, temperature, and protein concentrations. Results. A correlation between protein concentration in incubation media and percentage of specific binding of [H-3]-paroxetine was found: the lower the protein concentrations (10 and 20 mug/mL) in incubation media, the lower the percentage of specific paroxetine binding. Moreover, low specificity in [H-3]-paroxetine binding affected Bmax values obtained in saturation binding experiments. Conclusions. The use of low protein concentrations could affect Bmax values in binding assays of 5-HT uptake transporters. This may induce confusing interpretation of data in clinical experiments that use human platelets to explore the participation of serotonin in depressed patients. (C) 2003 IMSS. Published by Elsevier Inc.es_ES


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