Mostrar el registro sencillo del ítem

Real-Time Measurements of Synaptic Autoinhibition Produced by Serotonin Release in Cultured Leech Neurons

dc.creatorCercós, Montserrat G.
dc.creatorDe-Miguel, Francisco F.
dc.creatorTrueta, Citlali
dc.date.accessioned2017-06-30T03:46:44Z
dc.date.available2017-06-30T03:46:44Z
dc.date.issued2009es_ES
dc.identifier2250es_ES
dc.identifier.issn0022-3077es_ES
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/6897
dc.identifier.urihttps://doi.org/10.1152/jn.00107.2009es_ES
dc.language.isoenges_ES
dc.publisherAmerican Physiological Societyes_ES
dc.relation102 (2) 1075-1085 p.es_ES
dc.relationversión del editores_ES
dc.rightsacceso cerradoes_ES
dc.titleReal-Time Measurements of Synaptic Autoinhibition Produced by Serotonin Release in Cultured Leech Neuronses_ES
dc.typearticlees_ES
dc.contributor.affiliationDepartamento de Neurofisiología, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz. Distrito Federal, México.es_ES
dc.contributor.emailctrueta@imp.edu-mxes_ES
dc.relation.jnabreviadoJ NEUROPHYSIOL.es_ES
dc.relation.journalJournal of Neurophysiologyes_ES
dc.identifier.placeRockville Pike, Bethesda MDes_ES
dc.date.published2009es_ES
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñizes_ES
dc.identifier.eissn1522-1598es_ES
dc.identifier.doihttp://dx.doi.org/10.1152/jn.00107.2009es_ES
dc.description.monthAgoes_ES
dc.description.abstractotrodiomaWe studied autoinhibition produced immediately after synaptic serotonin (5-HT) release in identified leech Retzius neurons, cultured singly or forming synapses onto pressure-sensitive neurons. Cultured Retzius neurons are isopotential, thus allowing accurate recordings of synaptic events using intracellular microelectrodes. The effects of autoinhibition on distant neuropilar presynaptic endings were predicted from model simulations. Following action potentials (APs), cultured neurons produced a slow hyperpolarization with a rise time of 85.4 _ 5.2 ms and a half-decay time of 252 _ 17.4 ms. These inhibitory postpotentials were reproduced by the iontophoretic application of 5-HT and became depolarizing after inverting the transmembranal chloride gradient by using microelectrodes filled with potassium chloride. The inhibitory postpotentials were reversibly abolished in the absence of extracellular calcium and absent in reserpine-treated neurons, suggesting an autoinhibition due to 5-HT acting on autoreceptors coupled to chloride channels. The autoinhibitory responses increased the membrane conductance and decreased subsequent excitability. Increasing 5-HT release by stimulating with trains of ten pulses at 10 or 30 Hz produced 23 _ 6 and 47 _ 2% of AP failures, respectively. These failures were reversibly abolished by the serotonergic antagonist methysergide (140 _M). Moreover, reserpine-treated neurons had only 5 _ 4% of failures during trains at 10 Hz. This percentage was increased to 35 _ 4% by iontophoretic application of 5-HT. Increases in AP failures correlated with smaller postsynaptic currents. Model simulations predicted that the autoinhibitory chloride conductance reduces the amplitude of APs arriving at neuropilar presynaptic endings. Altogether, our results suggest that 5-HT autoinhibits its subsequent release by decreasing the excitability of presynaptic endings within the same neuron.es_ES
dc.subject.kwAutoinhibiciónes_ES
dc.subject.kwSerotonina sinápticaes_ES
dc.subject.kwSinapsises_ES
dc.subject.kwSerotininaes_ES
dc.subject.koAutoinhibitiones_ES
dc.subject.koSynaptic serotonines_ES
dc.subject.koSynapsees_ES
dc.subject.koSerotonines_ES


Ficheros en el ítem

FicherosTamañoFormatoVer

No hay ficheros asociados a este ítem.

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem