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dc.creatorRamírez-Rodríguez, Gerardo
dc.creatorBabu, Harish
dc.creatorKlempin, Friederike
dc.creatorKrylyshkina, Olga
dc.creatorBaekelandt, Veerle
dc.creatorGijsbers, Rik
dc.creatorDebyser, Zeger
dc.creatorOverall, Rupert
dc.creatorNicola, Zeina
dc.creatorFabel, Klaus
dc.creatorKempermann, Gerd
dc.date.accessioned2017-06-30T03:44:02Z
dc.date.available2017-06-30T03:44:02Z
dc.date.issued2013es_ES
dc.identifier2193es_ES
dc.identifier.issn0270-6474es_ES
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/6840
dc.identifier.urihttps://doi.org/10.1523/JNEUROSCI.6452-11.2013es_ES
dc.identifier.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6705050/es_ES
dc.description.abstractAdult hippocampal neurogenesis is to a large degree controlled at the level of cell survival, and a number of potential mediators of this effect have been postulated. Here, we investigated the small heat shock protein Hspb8, which, because of its pleiotropic prosurvival effects in other systems, was considered a particularly promising candidate factor. Hspb8 is, for example, found in plaques of Alzheimer disease but exerts neuroprotective effects. We found that expression of Hspb8 increased during differentiation in vitro and was particularly associated with later stages (48–96 h) of differentiation. Gain-of-function and loss-of-function experiments supported the hypothesis that Hspb8 regulates cell survival of new neurons in vitro. In the dentate gyrus of adult mice in vivo, lentiviral overexpression of Hspb8 doubled the surviving cells and concomitantly promoted differentiation and net neurogenesis without affecting precursor cell proliferation. We also discovered that the truncated form of the crystallin domain of Hspb8 was sufficient to affect cell survival and neuronal differentiation in vitro and in vivo. Precursor cell experiments in vitro revealed that Hspb8 increases the phosphorylation of Akt and suggested that the prosurvival effect can be produced by a cell-autonomous mechanism. Analysis of hippocampal Hspb8 expression in mice of 69 strains of the recombinant inbred set BXD revealed that Hspb8 is a cis-acting gene whose expression was associated with clusters of transcript enriched in genes linked to growth factor signaling and apoptosis. Our results strongly suggest that Hspb8 and its _-crystallin domain might act as pleiotropic prosurvival factor in the adult hippocampus.es_ES
dc.language.isoenges_ES
dc.relation33 (13) 5785-5796 p.es_ES
dc.relationversión del editores_ES
dc.rightsacceso cerradoes_ES
dc.titleThe α Domain of Small Heat Shock Protein b8 (Hspb8) Acts as Survival and Differentiation Factor in Adult Hippocampal Neurogenesises_ES
dc.typearticlees_ES
dc.contributor.affiliationInstituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, Calz, México-Xochimilco 101, Col. Sn Lorenzo Huipulco 14370, México, D.F., Mexicoes_ES
dc.contributor.emailgerd.kempermann@crt-dresden.dees_ES
dc.relation.jnabreviadoJ NEUROSCIes_ES
dc.relation.journalThe Journal of neurosciencees_ES
dc.identifier.placeWashington, DCes_ES
dc.date.published2013es_ES
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, México.es_ES
dc.identifier.eissn1529-2401es_ES
dc.identifier.doi10.1523/JNEUROSCI.6452-11.2013es_ES


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