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A helminth cestode parasite express an estrogen-binding protein resembling a classic nuclear estrogen receptor

dc.creatorIbarra-Coronado, Elizabeth Guadalupe
dc.creatorEscobedo, Galileo
dc.creatorNava-Castro, Karen
dc.creatorChávez-Rios, Jesús Ramses
dc.creatorHernández-Bello, Romel
dc.creatorGarcía-Varela, Martín
dc.creatorAmbrosio, Javier R.
dc.creatorReynoso-Ducoing, Olivia
dc.creatorFonseca-Liñán, Rocío
dc.creatorOrtega-Pierres, Guadalupe
dc.creatorPavón, Lenin
dc.creatorHernández, María Eugenia
dc.creatorMorales-Montor, Jorge
dc.date.accessioned2017-06-30T03:43:09Z
dc.date.available2017-06-30T03:43:09Z
dc.date.issued2011es_ES
dc.identifier2168es_ES
dc.identifier.issn0039-128Xes_ES
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/6817
dc.identifier.urihttps://doi.org/10.1016/j.steroids.2011.05.003es_ES
dc.language.isoenges_ES
dc.publisherELSEVIER SCIENCE INC, 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USAes_ES
dc.relation76 (10-11) 1149-1159 p.es_ES
dc.relationversión del editores_ES
dc.rightsacceso cerradoes_ES
dc.subject.meshTaenia-crassicepses_ES
dc.subject.meshOnchocera-volvuluses_ES
dc.subject.meshSchistosoma-mansonies_ES
dc.subject.meshC-Foses_ES
dc.subject.meshParamyosines_ES
dc.subject.meshCysticercosises_ES
dc.subject.meshResponseses_ES
dc.subject.meshHostes_ES
dc.subject.meshReproductiones_ES
dc.subject.meshInfectionses_ES
dc.titleA helminth cestode parasite express an estrogen-binding protein resembling a classic nuclear estrogen receptores_ES
dc.typearticlees_ES
dc.contributor.affiliationUniv Nacl Autonoma Mexico, Inst Invest Biomed, Dept Inmunol, AP 70228, Mexico City 04510, DF, Mexico.es_ES
dc.contributor.emailjmontor66@biomedicas.unam.mxes_ES
dc.relation.jnabreviadoSTEROIDSes_ES
dc.relation.journalSteroidses_ES
dc.identifier.placeNew York, NYes_ES
dc.date.published2011es_ES
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, México.es_ES
dc.identifier.eissn1878-5867es_ES
dc.identifier.doi10.1016/j.steroids.2011.05.003   es_ES
dc.description.monthSep-Octes_ES
dc.description.abstractotrodiomaThe role of an estrogen-binding protein similar to a known mammalian estrogen receptor (ER) is described in the estradiol-dependent reproduction of the helminth parasite Taenia crassiceps. Previous results have shown that 17-beta-estradiol induces a concentration-dependent increase in bud number of in vitro cultured cysticerci. This effect is inhibited when parasites are also incubated in the presence of an ER binding-inhibitor (tamoxifen). RT-PCR assays using specific oligonucleotides of the most conserved ER sequences, showed expression by the parasite of a mRNA band of molecular weight and sequence corresponding to an ER. Western blot assays revealed reactivity with a 66 kDa protein corresponding to the parasite ER protein. Tamoxifen treatment strongly reduced the production of the T. crassiceps ER-like protein. Antibody specificity was demonstrated by immunoprecipitating the total parasite protein extract with anti-ER-antibodies. Cross-contamination by host cells was discarded by flow cytometry analysis. ER was specifically detected on cells expressing paramyosin, a specific helminth cell marker. Parasite cells expressing the ER-like protein were located by confocal microscopy in the subtegumental tissue exclusively. Analysis of the ER-like protein by bidimensional electrophoresis and immunoblot identified a specific protein of molecular weight and isoelectric point similar to a vertebrates ER. Sequencing of the spot produced a small fragment of protein similar to the mammalian nuclear ER. Together these results show that T. crassiceps expresses an ER-like protein which activates the budding of T. crassiceps cysticerci in vitro. To the best of our knowledge, this is the first report of an ER-like protein in parasites. This finding may have strong implications in the fields of host-parasite co-evolution as well as in sex-associated susceptibility to this infection, and could be an important target for the design of new drugs.es_ES
dc.subject.kwReceptor de estrógeno nucleares_ES
dc.subject.kwEstradioles_ES
dc.subject.kwCressiceps Taeniaes_ES
dc.subject.kwCisticercoses_ES
dc.subject.kwParásitoes_ES
dc.subject.kwReceptores hormonaleses_ES
dc.subject.koNuclear estrogen receptores_ES
dc.subject.koEstradioles_ES
dc.subject.koTaenia cressicepses_ES
dc.subject.koCysticercuses_ES
dc.subject.koParasitees_ES
dc.subject.koHormone receptorses_ES


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