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Melatonin activates PKC-alpha but not PKC-epsilon in N1E-115 cells

dc.creatorBenítez-King, Gloria
dc.creatorHernández, María Eugenia
dc.creatorTovar, Rosalinda
dc.creatorRamírez, Gerardo
dc.date.accessioned2017-06-30T03:42:30Z
dc.date.available2017-06-30T03:42:30Z
dc.date.issued2001es_ES
dc.identifier2144es_ES
dc.identifier.issn0197-0186es_ES
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/6799
dc.identifier.urihttps://doi.org/10.1016/S0197-0186(01)00021-3es_ES
dc.language.isoenges_ES
dc.relation39, 95-102 p.es_ES
dc.relationversión del editores_ES
dc.rightsacceso cerradoes_ES
dc.titleMelatonin activates PKC-alpha but not PKC-epsilon in N1E-115 cellses_ES
dc.typearticlees_ES
dc.contributor.affiliationDepartamento de Neurofarmacolog?a, SIC, Instituto Nacional de Psiquiatr?a, Ramon de la Fuente Muñiz, Calz Mexico-Xochimilco No 101, CP 14370, Col. San Lorenzo Huipulco, D.F., Mexicoes_ES
dc.contributor.emailbekin@imp.edu.mxes_ES
dc.relation.jnabreviadoNEUROCHEM INTes_ES
dc.relation.journalNeurochemistry Internationales_ES
dc.date.published2001es_ES
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, México.es_ES
dc.identifier.eissn1872-9754es_ES
dc.description.monthFebes_ES
dc.description.abstractotrodiomaPrevious reports have revealed that calmodulin antagonism by melatonin is followed by microtubule enlargements and neurite outgrowths in neuroblastoma N1E-115 cells. In addition, activation of protein kinase C (PKC) by this neurohormone is also followed by increased vimentin phosphorylation, and reorganization of vimentin intermediate filaments (IFs) in N1E-115 cells. In this work, we further characterize the activation of PKC by melatonin in neuroblastoma N1E-115 cells. We studied the Ca2+-dependent effects of melatonin on PKC activity and distribution of PKC-? in isolated N1E-115 cell IFs. Also, the effects of melatonin on PKC-? translocation in comparison to PKC-?, were studied in intact N1E-115 cells. The results showed that both melatonin and the PKC agonist phorbol-12-myristate-13-acetate increased PKC activity in isolated IFs. The effects of the hormone were Ca2+-dependent, while those caused by the phorbol ester were produced with or without Ca2+. Also, in isolated in situ IFs, the hormone changed the distribution of PKC-?. In intact N1E-115 cells, melatonin elicited PKC-? translocation and no changes were detected in PKC-?. Phorbol-12-myristate-13-acetate modified the subcellular distribution of both PKC isoforms. The results showed that melatonin selectively activates the Ca2+-dependent ? isoform of PKC and suggest that PKC-? activation by melatonin underlies IF rearrangements and participates in neurite formation in N1E-115 cells.es_ES
dc.subject.kwMelatoninaes_ES
dc.subject.kwProteína quinasa Ces_ES
dc.subject.kwFilamentos intermedioses_ES
dc.subject.kwVimentinaes_ES
dc.subject.kwCitoesqueletoes_ES
dc.subject.koMelatonines_ES
dc.subject.koProtein kinase Ces_ES
dc.subject.koIntermediate filamentses_ES
dc.subject.koVimentines_ES
dc.subject.koCytoskeletones_ES


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