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Expression of the dopaminergic D1 and D2 receptors in the anterior cingulate cortex in a model of neuropathic pain

dc.creatorOrtega-Legaspi, J. Manuel
dc.creatorGortari, Patricia de
dc.creatorGarduño-Gutiérrez, René
dc.creatorAmaya, María Isabel
dc.creatorLeón-Olea, Martha
dc.creatorCoffeen, Ulises
dc.creatorPellicer, Francisco
dc.date.accessioned2017-06-30T02:06:02Z
dc.date.available2017-06-30T02:06:02Z
dc.date.issued2011es_ES
dc.identifier1520es_ES
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/6199
dc.identifier.urihttps://doi.org/10.1186/1744-8069-7-97es_ES
dc.identifier.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3286425/es_ES
dc.language.isoenges_ES
dc.relation7 (97) 1-10 p.es_ES
dc.relationversión del editores_ES
dc.rightsacceso cerradoes_ES
dc.titleExpression of the dopaminergic D1 and D2 receptors in the anterior cingulate cortex in a model of neuropathic paines_ES
dc.typearticlees_ES
dc.contributor.affiliationLaboratorio de Neurofisiología Integrativa, Dirección de Neurociencias, Instituto Nacional de Psiquiatría Ramón de la Fuente, México.es_ES
dc.contributor.emailpellicer@imp.edu.mxes_ES
dc.relation.jnabreviadoMOL PAINes_ES
dc.relation.journalMolecular Paines_ES
dc.identifier.placeInglaterraes_ES
dc.date.published2011es_ES
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñizes_ES
dc.identifier.eissn1744-8069es_ES
dc.identifier.doi10.1186/1744-8069-7-97es_ES
dc.description.monthDices_ES
dc.description.abstractotrodiomaBACKGROUND: The anterior cingulate cortex (ACC) has been related to the affective component of pain. Dopaminergic mesocortical circuits, including the ACC, are able to inhibit neuropathic nociception measured as autotomy behaviour. We determined the changes in dopamine D1 and D2 (D1R and D2R) receptor expression in the ACC (cg1 and cg2) in an animal model of neuropathic pain. The neuropathic group had noxious heat applied in the right hind paw followed 30 min. Later by right sciatic denervation. Autotomy score (AS) was recorded for eight days and subsequently classified in low, medium and high AS groups. The control consisted of naïve animals.A semiquantitative RT-PCR procedure was done to determine mRNA levels for D1R and D2R in cg1 and cg2, and protein levels were measured by Western Blot. RESULTS: The results of D1R mRNA in cg1 showed a decrease in all groups. D2R mRNA levels in cg1 decreased in low AS and increased in medium and high AS. Regarding D1R in cg2, there was an increase in all groups. D2R expression levels in cg2 decreased in all groups. In cg1, the D2R mRNA correlated positively with autotomy behaviour. Protein levels of D2R in cg1 increased in all groups but to a higher degree in low AS. In cg2 D2R protein only decreased discretely. D1R protein was not found in either ACC region. CONCLUSIONS: This is the first evidence of an increase of inhibitory dopaminergic receptor (D2R) mRNA and protein in cg1 in correlation with nociceptive behaviour in a neuropathic model of pain in the rates_ES
dc.subject.koAnimalses_ES
dc.subject.koGene Expressiones_ES
dc.subject.koGyrus Cingulies_ES
dc.subject.kometabolismes_ES
dc.subject.koMalees_ES
dc.subject.koNeuralgiaes_ES
dc.subject.kogeneticses_ES
dc.subject.koNeuralgiaes_ES
dc.subject.kometabolismes_ES
dc.subject.koRNA, Messengeres_ES
dc.subject.kometabolismes_ES
dc.subject.koRatses_ES
dc.subject.koRats, Wistares_ES
dc.subject.koReceptors, Dopamine D1es_ES
dc.subject.kogeneticses_ES
dc.subject.koReceptors, Dopamine D1es_ES
dc.subject.kometabolismes_ES
dc.subject.koReceptors, Dopamine D2es_ES
dc.subject.kogeneticses_ES
dc.subject.koReceptors, Dopamine D2es_ES
dc.subject.kometabolismes_ES
dc.subject.koRNA, Messengeres_ES
dc.subject.koReceptors, Dopamine D1es_ES
dc.subject.koReceptors, Dopamine D2es_ES


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