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Amygdala kindling differentially regulates the expression of the elements involved in TRH transmission

dc.creatorDe Gortari, P.
dc.creatorUribe, R.M.
dc.creatorGarcía-Vázquez, A.
dc.creatorAguilar-Valles, A.
dc.creatorMartínez, A.
dc.creatorValdés, A.
dc.creatorCharli, J.L.
dc.creatorFernández-Guardiola, A.
dc.creatorJoseph-Bravo, P.
dc.date.accessioned2017-06-30T01:34:14Z
dc.date.available2017-06-30T01:34:14Z
dc.date.issued2006es_ES
dc.identifier1466es_ES
dc.identifier.issn0197-0186es_ES
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/6147
dc.identifier.urihttps://doi.org/10.1016/j.neuint.2005.08.003es_ES
dc.language.isoenges_ES
dc.relation48 (1) 31-42 p.es_ES
dc.relationversión del editores_ES
dc.rightsacceso cerradoes_ES
dc.subject.meshAmygdala-Physiologyes_ES
dc.subject.meshAnimalses_ES
dc.subject.meshBase Sequencees_ES
dc.subject.meshDNA Primerses_ES
dc.subject.meshGene Expression Regulation-Physiologyes_ES
dc.subject.meshKindling, Neurologices_ES
dc.subject.meshMalees_ES
dc.subject.meshRNA, Messenger-Geneticses_ES
dc.subject.meshRNA, Messenger-Metabolismes_ES
dc.subject.meshRatses_ES
dc.subject.meshRats, Wistares_ES
dc.subject.meshReceptors, Thyrotropin-Releasing Hormone-Geneticses_ES
dc.subject.meshReverse Transcriptase Polymerase Chain Reactiones_ES
dc.subject.meshThyrotropin-Releasing Hormone-Physiologyes_ES
dc.subject.meshDNA Primerses_ES
dc.subject.meshRNA, Messengeres_ES
dc.subject.meshReceptors, Thyrotropin-Releasing Hormonees_ES
dc.subject.meshThyrotropin-Releasing Hormonees_ES
dc.titleAmygdala kindling differentially regulates the expression of the elements involved in TRH transmissiones_ES
dc.typearticlees_ES
dc.contributor.affiliationDept. Neurociencias, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñíz, Czda. México-Xochimilco 102, Sn. Lorenzo Huipulco, México D.F. 14370, Mexicoes_ES
dc.contributor.emailjoseph@ibt.unam.mxes_ES
dc.relation.jnabreviadoNEUROCHEM INTes_ES
dc.relation.journalNeurochemistry Internationales_ES
dc.identifier.placeInglaterraes_ES
dc.date.published2006es_ES
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñizes_ES
dc.identifier.eissn1872-9754es_ES
dc.identifier.doi10.1016/j.neuint.2005.08.003es_ES
dc.description.monthEnees_ES
dc.description.abstractotrodiomaSubthreshold electrical stimulation of the amygdala (kindling) activates neuronal pathways increasing the expression of several neuropeptides including thyrotropin releasing-hormone (TRH). Partial kindling enhances TRH expression and the activity or its inactivating ectoenzyme; once kindling is established (stage V), TRH and its mRNA levels are further increased but TRH-binding and pyroglutamyl aminopeptidase II (PPII) activity decreased in epileptogenic areas. To determine whether variations in TRH receptor binding or PPII activity are due to regulation of their synthesis, mRNA levels of TRH receptors (R1, R2) and PPII were semi-quantified by RT-PCR in amygdala, frontal cortex and hippocampus of kindled rats sacrificed at stage II or V. Increased mRNA levels of PPII were found at stage II in amygdala and frontal cortex, and of pro-TRH and TRH-R2, in amygdala and hippocampus. At stage V, pro-TRH mRNA levels increased and those of PPII, decreased in the three regions; TRH-R2 mRNA levels diminished in amygdala and frontal cortex and of TRH-R1 only in amygdala. In situ hybridization analyses revealed, at stage II, enhanced TRH-R1 mRNA levels in dentate gyrus and amygdala while decreased in piriform cortex; those of TRH-R2 increased in amygdala, CA2, dentate gyrus, piriform cortex, thalamus and subiculum and of PPII, in CAs and piriform cortex. In contrast, at stage V decreased expression of TRH-R1 occurred in amygdala, CA2-3, dentate gyrus and piriform cortex; of TRH-R2 in CA2, thalamus and piriform cortex, and of PPII in CA2, and amygdala. The magnitude of changes differed between ipsi and contralateral side. These results support a trans-synaptic modulation of all elements involved in TRH transmission in conditions that stimulate the activity of TRHergic neurons. They show that reported changes in PPII activity or TRH-binding caused by kindling relate to regulation of the expression of TRH receptors and degrading enzymees_ES
dc.subject.kwTirotropina hormona liberadoraes_ES
dc.subject.kwTHL R1es_ES
dc.subject.kwTHL R2es_ES
dc.subject.kwPiroglutamil aminopeptidasa IIes_ES
dc.subject.kwAstillases_ES
dc.subject.koTRHes_ES
dc.subject.koTRH-R1es_ES
dc.subject.koTRH-R2es_ES
dc.subject.koPro-TRH mRNAes_ES
dc.subject.koPyroglutamyl aminopeptidase IIes_ES
dc.subject.koKindlinges_ES


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