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Analysis of the stress response in rats trained in the water-maze: differential expression of corticotropin-releasing hormone, CRH-R1, glucocorticoid receptors and brain-derived neurotrophic factor in limbic regions

dc.creatorAguilar-Valles, Argel
dc.creatorSánchez, Edith
dc.creatorDe Gortari, Patricia
dc.creatorBalderas, Israela
dc.creatorRamírez-Amaya, Víctor
dc.creatorBermúdez-Rattoni, Federico
dc.creatorJoseph-Bravo, Patricia
dc.date.accessioned2017-06-30T01:32:43Z
dc.date.available2017-06-30T01:32:43Z
dc.date.issued2005es_ES
dc.identifier1454es_ES
dc.identifier.issn0028-3835es_ES
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/6135
dc.identifier.urihttps://doi.org/10.1159/000093129es_ES
dc.language.isoenges_ES
dc.relation82 (5-6) 306-319 p.es_ES
dc.relationversión del editores_ES
dc.rightsacceso cerradoes_ES
dc.titleAnalysis of the stress response in rats trained in the water-maze: differential expression of corticotropin-releasing hormone, CRH-R1, glucocorticoid receptors and brain-derived neurotrophic factor in limbic regionses_ES
dc.typearticlees_ES
dc.contributor.affiliationDepartamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México (UNAM), México.es_ES
dc.contributor.emailjoseph@ibt.unam.mxes_ES
dc.relation.jnabreviadoNEUROENDOCRINOLOGYes_ES
dc.relation.journalNeuroendocrinologyes_ES
dc.identifier.placeSuizaes_ES
dc.date.published2005es_ES
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñizes_ES
dc.identifier.eissn1423-0194es_ES
dc.identifier.doi10.1159/000093129es_ES
dc.description.abstractotrodiomaGlucocorticoids and corticotropin-releasing hormone (CRH) are key regulators of stress responses. Different types of stress activate the CRH system; in hypothalamus, CRH expression and release are increased by physical or psychological stressors while in amygdala, preferentially by psychological stress. Learning and memory processes are modulated by glucocorticoids and stress at different levels. To characterize the kind of stress provoked by a hippocampal-dependent task such as spatial learning, we compared the expression profile of glucocorticoid receptor (GR), pro-CRH and CRH-R1 mRNAs (analyzed by RT-PCR), in amygdala, hippocampus and hypothalamus and quantified serum corticosterone levels by radioimmunoassay at different stages of training. mRNA levels of brain-derived neurotrophic factor (BDNF) were also quantified due to its prominent role in learning and memory processes. Male Wistar rats trained for 1, 3 or 5 days in the Morris water-maze (10 trials-day) were sacrificed 5-60 min the after last trial. A strong stress response occurred at day one in both yoked and trained animals (increased corticosterone and hypothalamic pro-CRH and CRH-R1 mRNA levels); changes gradually diminished as the test progressed. In amygdala, pro-CRH mRNA levels decreased while those of BDNF augmented when stress was highest, in yoked and trained animals. Hippocampi, of both yoked and trained groups, had decreased levels of GR mRNA on days 1 and 3, normalizing by day 5, while those of pro-CRH and CRH-R1 increased after the 3rd day. Increased gene expression, specifically due to spatial learning, occurred only for hippocampal BDNF since day 3. These results show that the Morris water-maze paradigm induces a strong stress response that is gradually attenuated. Inhibition of CRH expression in amygdala suggests that the stress inflicted is of physical but not of psychological nature and could lead to reduced fear or anxietyes_ES
dc.subject.koAmygdalaes_ES
dc.subject.kochemistryes_ES
dc.subject.koAmygdalaes_ES
dc.subject.kophysiologyes_ES
dc.subject.koAnimalses_ES
dc.subject.koAnxietyes_ES
dc.subject.kophysiopathologyes_ES
dc.subject.koBrain-Derived Neurotrophic Factores_ES
dc.subject.koanalysises_ES
dc.subject.koBrain-Derived Neurotrophic Factores_ES
dc.subject.kogeneticses_ES
dc.subject.koBrain-Derived Neurotrophic Factores_ES
dc.subject.kophysiologyes_ES
dc.subject.koCorticosteronees_ES
dc.subject.kobloodes_ES
dc.subject.koCorticotropin-Releasing Hormonees_ES
dc.subject.koanalysises_ES
dc.subject.koCorticotropin-Releasing Hormonees_ES
dc.subject.kogeneticses_ES
dc.subject.koCorticotropin-Releasing Hormonees_ES
dc.subject.kophysiologyes_ES
dc.subject.koGene Expression Regulationes_ES
dc.subject.kophysiologyes_ES
dc.subject.koHippocampuses_ES
dc.subject.kochemistryes_ES
dc.subject.koHippocampuses_ES
dc.subject.kophysiologyes_ES
dc.subject.koHypothalamo-Hypophyseal Systemes_ES
dc.subject.kophysiologyes_ES
dc.subject.koHypothalamuses_ES
dc.subject.kochemistryes_ES
dc.subject.koHypothalamuses_ES
dc.subject.kophysiologyes_ES
dc.subject.koIn Situ Hybridizationes_ES
dc.subject.koLimbic Systemes_ES
dc.subject.kochemistryes_ES
dc.subject.koLimbic Systemes_ES
dc.subject.kophysiologyes_ES
dc.subject.koMalees_ES
dc.subject.koMaze Learninges_ES
dc.subject.kophysiologyes_ES
dc.subject.koPituitary-Adrenal Systemes_ES
dc.subject.kophysiologyes_ES
dc.subject.koRNA, Messengeres_ES
dc.subject.koanalysises_ES
dc.subject.koRNA, Messengeres_ES
dc.subject.kogeneticses_ES
dc.subject.koRatses_ES
dc.subject.koRats, Wistares_ES
dc.subject.koReceptors, Corticotropin-Releasing Hormonees_ES
dc.subject.koanalysises_ES
dc.subject.koReceptors, Corticotropin-Releasing Hormonees_ES
dc.subject.kogeneticses_ES
dc.subject.koReceptors, Corticotropin-Releasing Hormonees_ES
dc.subject.kophysiologyes_ES
dc.subject.koReceptors, Glucocorticoides_ES
dc.subject.koanalysises_ES
dc.subject.koReceptors, Glucocorticoides_ES
dc.subject.kogeneticses_ES
dc.subject.koReceptors, Glucocorticoides_ES
dc.subject.kophysiologyes_ES
dc.subject.koReverse Transcriptase Polymerase Chain Reactiones_ES
dc.subject.koSpatial Behaviores_ES
dc.subject.kophysiologyes_ES
dc.subject.koStress, Physiologicales_ES
dc.subject.koetiologyes_ES
dc.subject.koStress, Physiologicales_ES
dc.subject.kophysiopathologyes_ES
dc.subject.koTime Factorses_ES
dc.subject.koWateres_ES
dc.subject.koBrain-Derived Neurotrophic Factores_ES
dc.subject.koCRF receptor type 1es_ES
dc.subject.koRNA, Messengeres_ES
dc.subject.koReceptors, Corticotropin-Releasing Hormonees_ES
dc.subject.koReceptors, Glucocorticoides_ES
dc.subject.koCorticosteronees_ES
dc.subject.koWateres_ES
dc.subject.koCorticotropin-Releasing Hormonees_ES


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