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Comparison of the effects of Percoll and PureSperm on the common marmoset (Callithrix jacchus) semen

dc.creatorHernández-López, L.
dc.creatorUmland, N.
dc.creatorMondragón-Ceballos, R.
dc.creatorNayudu, P.L.
dc.date.accessioned2017-06-30T01:32:25Z
dc.date.available2017-06-30T01:32:25Z
dc.date.issued2005es_ES
dc.identifier1452es_ES
dc.identifier.issn0047-2565es_ES
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/6133
dc.identifier.urihttps://doi.org/10.1111/j.1600-0684.2005.00095.xes_ES
dc.language.isoenges_ES
dc.relation34 (2) 86-90 p.es_ES
dc.relationversión del editores_ES
dc.rightsacceso cerradoes_ES
dc.titleComparison of the effects of Percoll and PureSperm on the common marmoset (Callithrix jacchus) semenes_ES
dc.typearticlees_ES
dc.contributor.affiliationDepartment of Reproductive Biology, German Primate Center, Goettingen, Germanyes_ES
dc.contributor.emaillhl@imp.edu.mxes_ES
dc.relation.jnabreviadoJ MED PRIMATOLes_ES
dc.relation.journalJournal of Medical Primatologyes_ES
dc.identifier.placeDinamarcaes_ES
dc.date.published2005es_ES
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñizes_ES
dc.identifier.eissn1600-0684es_ES
dc.identifier.doi10.1111/j.1600-0684.2005.00095.xes_ES
dc.description.monthAbres_ES
dc.description.abstractotrodiomaDensity gradient centrifugation is a widely used technique for the separation of motile from non-motile sperm, for the removal of contaminating agents such as bacteria and viruses, and for the removal of seminal plasma or cryoprotectant. In the choice of a density gradient medium for a new species, it is important to perform toxicity tests. The present study was carried out to evaluate the potential toxic effects of two silica-based density gradient products (Percoll and PureSperm), on the sperm of the common marmoset. We assessed two different batches of Percoll (polyvinylpyrrolidone (PVP)-coated colloidal silica particles) and one of PureSperm (saline-coated colloidal silica suspension) by means of a computer-aided sperm analysis on semen collected by vibrostimulation. The results showed that although some of the sperm patterns of movement and viability changed significantly over time, and provide a first description of marmoset sperm motility changes under capacitating conditions, there was no significant difference in the sperm treated with Percoll or PureSperm in comparison with the control. We conclude that simple exposure to either of these products does not have a negative effect on viability or motility of marmoset spermes_ES
dc.subject.koAnimalses_ES
dc.subject.koCallithrixes_ES
dc.subject.koCell Separationes_ES
dc.subject.komethodses_ES
dc.subject.koCell Separationes_ES
dc.subject.koveterinaryes_ES
dc.subject.koCell Survivales_ES
dc.subject.kodrug effectses_ES
dc.subject.koCentrifugation, Density Gradientes_ES
dc.subject.koveterinaryes_ES
dc.subject.koColloidses_ES
dc.subject.koIndicators and Reagentses_ES
dc.subject.kotoxicityes_ES
dc.subject.koMalees_ES
dc.subject.koPovidonees_ES
dc.subject.kotoxicityes_ES
dc.subject.koSemenes_ES
dc.subject.kocytologyes_ES
dc.subject.koSilicon Dioxidees_ES
dc.subject.kotoxicityes_ES
dc.subject.koSperm Capacitationes_ES
dc.subject.koSperm Motilityes_ES
dc.subject.kodrug effectses_ES
dc.subject.koSpermatozoaes_ES
dc.subject.kodrug effectses_ES
dc.subject.koColloidses_ES
dc.subject.koIndicators and Reagentses_ES
dc.subject.koPercolles_ES
dc.subject.koSilicon Dioxidees_ES
dc.subject.koPovidonees_ES


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