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Maitotoxin induces two dose-dependent conductances in Xenopus oocytes. Comparison with nystatin effects as a pore inductor

dc.creatorEgido, W.
dc.creatorCastrejón, V.
dc.creatorAntón, B.
dc.creatorMartínez, M.
dc.date.accessioned2017-06-29T06:04:25Z
dc.date.available2017-06-29T06:04:25Z
dc.date.issued2008es_ES
dc.identifier611es_ES
dc.identifier.issn0041-0101es_ES
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/5295
dc.identifier.urihttps://doi.org/10.1016/j.toxicon.2007.12.013es_ES
dc.language.isoenges_ES
dc.relation51 (5) 797-812 p.es_ES
dc.relationversión del editores_ES
dc.rightsacceso cerradoes_ES
dc.subject.meshAnimalses_ES
dc.subject.meshCell Membranees_ES
dc.subject.meshDose-Response Relationship, Druges_ES
dc.subject.meshElectric Conductivityes_ES
dc.subject.meshIon Channels-agonistses_ES
dc.subject.meshLanthanum-toxicityes_ES
dc.subject.meshMarine Toxins-toxicityes_ES
dc.subject.meshMembrane Potentialses_ES
dc.subject.meshNystatin-toxicityes_ES
dc.subject.meshOocytes-drug effectses_ES
dc.subject.meshOocytes-metabolismes_ES
dc.subject.meshOxocins-toxicityes_ES
dc.subject.meshXenopuses_ES
dc.titleMaitotoxin induces two dose-dependent conductances in Xenopus oocytes. Comparison with nystatin effects as a pore inductores_ES
dc.typearticlees_ES
dc.contributor.affiliationLaboratorio de Cronobiología, Departamento de Fisiología, Facultad de Medicina, UNAM, Mexico.es_ES
dc.relation.jnabreviadoTOXICONes_ES
dc.relation.journalToxicones_ES
dc.identifier.placeOxford ; New Yorkes_ES
dc.date.published2008es_ES
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñizes_ES
dc.identifier.eissn1879-3150es_ES
dc.identifier.doi10.1016/j.toxicon.2007.12.013es_ES
dc.description.monthAbres_ES
dc.description.abstractotrodiomaMaitotoxin (MTX)-induced conductances in Xenopus oocytes were thoroughly characterized using the two-electrode voltage clamp technique with a hyperpolarizing voltage protocol. MTX 5-100pM induced an inward current with maximal amplitude between 0.1 and 10microA. The kinetics of this current had rising and decaying phases, which were non-voltage dependent. Its reversal potential (Erev) was close to 0mV in high K+ or Na+ external solution, indicating the participation of non-selective cation channels (NSCC). A second conductance was developed at MTX doses higher than 200pM whose amplitude increased continuously. This current showed a large instantaneous component and a voltage-independent decay, as well as similar selectivity for Na+ and K+ ions (Erev approximately 0 mV). Moreover, the maximal current amplitude was about 34% bigger in high K+ than in high Na+. The MTX effect was reversible at all doses in pM range. All the properties found are similar to those of NSCC. The differences in the current kinetics suggest that the MTX-elicited currents reflect the activation of two sets of voltage-independent NSCC. As MTX has been proposed to act by forming pores directly into the plasma membrane, we compared its effects with those of nystatin, a well-known membrane pore inductor. We found strong differences between the effects of both substances suggesting different mechanisms for these drugs.es_ES


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