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Airway smooth muscle relaxation induced by 5-HT2A receptors: Role of Na+/K+-ATPase pump and Ca2+-activated K+ channels

dc.creatorCampos-Bedolla, Patricia
dc.creatorVargas, Mario H.
dc.creatorSegura, Patricia
dc.creatorCarbajal, Verónica
dc.creatorCalixto, Eduardo
dc.creatorFigueroa, Alejandra
dc.creatorFlores-Soto, Edgar
dc.creatorBarajas-López, Carlos
dc.creatorMendoza-Patiño, Nicandro
dc.creatorMontaño, Luis M.
dc.date.accessioned2017-06-29T06:03:12Z
dc.date.available2017-06-29T06:03:12Z
dc.date.issued2008es_ES
dc.identifier574es_ES
dc.identifier.issn0024-3205es_ES
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/5261
dc.identifier.urihttps://doi.org/10.1016/j.lfs.2008.07.006es_ES
dc.language.isoenges_ES
dc.relation83 (11-12) 438-446 p.es_ES
dc.relationversión del editores_ES
dc.rightsacceso cerradoes_ES
dc.titleAirway smooth muscle relaxation induced by 5-HT2A receptors: Role of Na+/K+-ATPase pump and Ca2+-activated K+ channelses_ES
dc.typearticlees_ES
dc.contributor.affiliationUnidad de Investigación Médica en Enfermedades Neurológicas, Hospital de Especialidades, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, México DF, Mexicoes_ES
dc.contributor.emaillmmr@unam.mxes_ES
dc.relation.jnabreviadoLIFE SCIes_ES
dc.relation.journalLife Scienceses_ES
dc.identifier.placeNetherlandses_ES
dc.date.published2008es_ES
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñizes_ES
dc.identifier.eissn1879-0631es_ES
dc.identifier.doi10.1016/j.lfs.2008.07.006es_ES
dc.description.abstractotrodiomaAimsAlthough 5-hydroxytryptamine (5-HT) contracts airway smooth muscle in many mammalian species, in guinea pig and human airways 5-HT causes a contraction followed by relaxation. This study explored potential mechanisms involved in the relaxation induced by 5-HT.Main methodsUsing organ baths, patch clamp, and intracellular Ca2+ measurement techniques, the effect of 5-HT on guinea pig airway smooth muscle was studied.Key findingsA wide range of 5-HT concentrations caused a biphasic response of tracheal rings. Response to 32 _M 5-HT was notably reduced by either tropisetron or methiothepin, and almost abolished by their combination. Incubation with 10 nM ketanserin significantly prevented the relaxing phase. Likewise, incubation with 100 nM charybdotoxin or 320 nM iberiotoxin and at less extent with 10 _M ouabain caused a significant reduction of the relaxing phase induced by 5-HT. Propranolol, L-NAME and 5-HT1A, 5-HT1B-5-HT1D and 5-HT2B receptors antagonist did not modify this relaxation. Tracheas from sensitized animals displayed reduced relaxation as compared with controls. In tracheas precontracted with histamine, a concentration response curve to 5-HT (32, 100 and 320 _M) induced relaxation and this effect was abolished by charybdotoxin, iberiotoxin or ketanserin. In single myocytes, 5-HT in the presence of 3 mM 4-AP notably increased the K+ currents (IK(Ca)), and they were completely abolished by charybdotoxin, iberiotoxin or ketanserin.SignificanceDuring the relaxation induced by 5-HT two major mechanisms seem to be involved: stimulation of the Na+-K+-ATPase pump, and increasing activity of the high-conductance Ca2+-activated K+ channels, probably via 5-HT2A receptors.es_ES
dc.subject.koAirway smooth musclees_ES
dc.subject.koRelaxationes_ES
dc.subject.ko5-HT2A receptorses_ES
dc.subject.koBKCa channelses_ES
dc.subject.koNa+es_ES
dc.subject.koK+-ATPase pumpes_ES


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