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Presence of pro-opiomelanocortin mRNA in the rat medial prefrontal cortex, nucleus accumbens and ventral tegmental area: studies by RT-PCR and in situ hybridization techniques

dc.creatorLeriche, M
dc.creatorCote-Vélez, A
dc.creatorMéndez, M.
dc.date.accessioned2017-06-29T06:02:08Z
dc.date.available2017-06-29T06:02:08Z
dc.date.issued2007es_ES
dc.identifier527es_ES
dc.identifier.issn0143-4179es_ES
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/5214
dc.identifier.urihttps://doi.org/10.1016/j.npep.2007.08.004es_ES
dc.language.isoenges_ES
dc.relation41 (6) 421-431 p.es_ES
dc.relationversión del editores_ES
dc.rightsacceso cerradoes_ES
dc.titlePresence of pro-opiomelanocortin mRNA in the rat medial prefrontal cortex, nucleus accumbens and ventral tegmental area: studies by RT-PCR and in situ hybridization techniqueses_ES
dc.typearticlees_ES
dc.contributor.affiliationInstituto Nacional de Psiquiatría Ramón de la Fuente, Calzada México-Xochimilco 101, Col. San Lorenzo Huipulco, 14370 México DF, Mexicoes_ES
dc.relation.jnabreviadoNEUROPEPTIDESes_ES
dc.relation.journalNeuropeptideses_ES
dc.identifier.placeEscociaes_ES
dc.date.published2007es_ES
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñizes_ES
dc.identifier.doi10.1016/j.npep.2007.08.004es_ES
dc.description.monthDices_ES
dc.description.abstractotrodiomaPro-opiomelanocortin (POMC) is a large proteic precursor which originates several biologically actives neuropeptides, such as beta-lipotropin (beta-LPH), beta-endorphin (beta-END), adenocorticotropic hormone (ACTH) and alpha-melanocyte-stimulating hormone (alpha-MSH). The arcuate nucleus of the hypothalamus is the main POMC producing cell group in brain and innervates several areas of the limbic system and brainstem. POMC-derived neuropeptides have been related to several motivated and rewarding behaviours, including sexual facilitation, feeding, and drug addiction. However, POMC mRNA has not been detected in regions of the dopaminergic mesocorticolimbic system, which represents the most important reward pathway. The aim of this work was to investigate if POMC mRNA is expressed in the medial prefrontal cortex (mPFC), the nucleus accumbens (NAcc) and the ventral tegmental area (VTA) of the rat. We used the reverse transcriptase reaction coupled to the polymerase chain reaction (RT-PCR). We also used the in situ hybridization technique to study the regional distribution of POMC mRNA in the same regions. We report that RT-PCR amplification of extracted RNA with two different pairs of primers generates the predicted 94bp and 678bp POMC-PCR products. Both the amplification of RNA obtained from the rat glial C-6 cell line (which does not express POMC mRNA) and the omission of reverse transcriptase from the RT reaction of rat brain samples showed no amplification products. We have shown for the first time that the rat medial prefrontal cortex, the nucleus accumbens and the ventral tegmental area contain POMC mRNA. This mRNA is in low concentration, ranging from 21% to 31% with respect to the hypothalamus. In situ hybridization experiments showed that POMC mRNA is homogeneously distributed in these areas. The presence of POMC mRNA in regions of the mesocorticolimbic system could have functional implications in motivated behaviours.es_ES


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