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Melatonin increases stress fibers and focal adhesions in MDCK cells: participation of Rho-associated kinase and protein kinase C

dc.creatorRamírez-Rodríguez, G.
dc.creatorOrtíz-López, L.
dc.creatorBenítez-King, G.
dc.date.accessioned2017-06-29T06:02:04Z
dc.date.available2017-06-29T06:02:04Z
dc.date.issued2007es_ES
dc.identifier523es_ES
dc.identifier.issn0742-3098es_ES
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/5210
dc.identifier.urihttps://doi.org/10.1111/j.1600-079X.2006.00404.xes_ES
dc.language.isoenges_ES
dc.relation42 (2) 180-190 p.es_ES
dc.relationversión del editores_ES
dc.rightsacceso cerradoes_ES
dc.subject.meshAnimalses_ES
dc.subject.meshCell Linees_ES
dc.subject.meshDogses_ES
dc.subject.meshFocal Adhesions-enzymologyes_ES
dc.subject.meshFocal Adhesions-metabolismes_ES
dc.subject.meshIntracellular Signaling Peptides and Proteins-physiologyes_ES
dc.subject.meshMelatonin-physiologyes_ES
dc.subject.meshProtein Kinase C-physiologyes_ES
dc.subject.meshProtein-Serine-Threonine Kinases-physiologyes_ES
dc.subject.meshStress Fibers-enzymologyes_ES
dc.subject.meshStress Fibers-metabolismes_ES
dc.subject.meshrho-Associated Kinaseses_ES
dc.titleMelatonin increases stress fibers and focal adhesions in MDCK cells: participation of Rho-associated kinase and protein kinase Ces_ES
dc.typearticlees_ES
dc.contributor.affiliationDepartamento de Neurofarmacología, Subdirección de Investigaciones Clínicas, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, Mexico City, Mexicoes_ES
dc.relation.jnabreviadoJ PINEAL RESes_ES
dc.relation.journalJournal of Pineal Researches_ES
dc.identifier.placeDenmarkes_ES
dc.date.published2007es_ES
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñizes_ES
dc.identifier.eissn1600-079Xes_ES
dc.identifier.doi10.1111/j.1600-079X.2006.00404.xes_ES
dc.description.monthMares_ES
dc.description.abstractotrodiomaMelatonin cyclically modifies water transport measured as dome formation in MDCK cells. An optimal increase in water transport, concomitant with elevated stress fiber (SF) formation, occurs at nocturnal plasma melatonin concentrations (1 nm) after 6 hr of incubation. Blockage in melatonin-elicited dome formation was observed with protein kinase C (PKC) inhibitors. Despite, this information on the precise mechanism by which melatonin increases SF formation involved in water transport is not known. Focal adhesion contacts (FAC) are cytoskeletal structures, which participate in MDCK membrane polarization. SF organization and vinculin phosphorylation are involved in FAC assembly and both processes are mediated by PKC, an enzyme stimulated by melatonin; in these processes also involved is Rho-associated kinase (ROCK). Thus, we studied FAC formation and the ROCK/PKC pathway as the mechanism by which melatonin increases SF formation and water transport. The results showed that 1 nM melatonin and the PKC agonist phorbol-12-miristate-13-acetate increased FAC. The PKC inhibitor GF109203x, and the ROCK inhibitor Y27632, blocked increased FAC caused by melatonin. ROCK and PKC activities, vinculin phosphorylation and FAC formation were increased with melatonin. The PKC inhibitor, GF109203x, abolished both melatonin stimulated FAC in whole cells and ROCK activity, indicating that ROCK is a downstream kinase in the melatonin-stimulated PKC pathway in MDCK cultured cells that causes an increase in SF and FAC formation. Data also document that melatonin modulates water transport through modifications of the cytoskeletal structurees_ES


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