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Melatonin induced cyclic modulation of vectorial water transport in kidney-derived MDCK cells

dc.creatorRamírez-Rodríguez, G.
dc.creatorMeza, I.
dc.creatorHernández, M.E.
dc.creatorCastillo, A.
dc.creatorBenítez-King, G.
dc.date.accessioned2017-06-29T04:30:28Z
dc.date.available2017-06-29T04:30:28Z
dc.date.issued2003es_ES
dc.identifier397es_ES
dc.identifier.issn0085-2538es_ES
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/5088
dc.identifier.urihttps://doi.org/10.1046/j.1523-1755.2003.00872.xes_ES
dc.language.isoenges_ES
dc.relation63 (4) 1356-1364 p.es_ES
dc.relationversión del editores_ES
dc.rightsacceso cerradoes_ES
dc.titleMelatonin induced cyclic modulation of vectorial water transport in kidney-derived MDCK cellses_ES
dc.typearticlees_ES
dc.contributor.affiliationDepartamento de Neurofarmacología, Subdirección de Investigaciones Clínicas, Instituto Nacional de Psiquiatría, Ramón de la Fuente Muñiz, México D.F. Méxicoes_ES
dc.relation.jnabreviadoKIDNEY INTes_ES
dc.relation.journalKidney Internationales_ES
dc.identifier.placeEstados Unidoses_ES
dc.date.published2003es_ES
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñizes_ES
dc.identifier.doi10.1046/j.1523-1755.2003.00872.xes_ES
dc.description.monthAbres_ES
dc.description.abstractotrodiomaBACKGROUND: Melatonin, newly synthesized by the pineal gland, is rapidly released to general circulation reaching a nanomolar concentration. Cyclic production of melatonin synchronizes body rhythms with the photoperiod. Moreover, changes in urine production and osmolarity have been observed in the kidney during the night. However, the precise mechanisms by which plasma-circulating melatonin modifies renal physiology are not clearly understood. METHODS: Madin-Darby canine kidney (MDCK) cell monolayers transport water vectorially from the apical to the basolateral side forming blisters or domes. Transport in epithelial cells is regulated by tight junction sealing, ion pumps and channels, and cytoskeleton organization, among other processes. MDCK cells were used to study vectorial water transport to determine the role of microfilament organization and protein kinase C (PKC) in dome formation in culture conditions that mimic the cyclic pattern of melatonin circulation in plasma. RESULTS: Melatonin cyclically increased dome formation by 50% and caused enlargement and thickening of stress fibers in cells surrounding the domes. Optimal increase in dome formation was observed at nanomolar concentrations of melatonin after 6 hours, concomitantly with a 28% decrease in the transepithelial electrical resistance, which remained low for up to 12 hours, without apparent change in fluorescein isothiocyanate (FITC)-dextran flux. A blockage in dome formation elicited by melatonin was observed in monolayers preincubated with the Na+-K+-ATPase or PKC inhibitors. CONCLUSION: The results obtained indicate that melatonin cyclically modifies the transepithelial permeability in kidney-derived cells through PKC activation and microfilament reorganization, and supports the hypothesis that melatonin may synchronize daily body rhythms through cyclic cytoskeletal rearrangements.es_ES
dc.subject.meshmActinses_ES
dc.subject.meshmAnimalses_ES
dc.subject.meshmAntioxidants-Pharmacologyes_ES
dc.subject.meshmCell Linees_ES
dc.subject.meshmCircadian Rhythm-Physiologyes_ES
dc.subject.meshmDose-Response Relationshipes_ES
dc.subject.meshmDruges_ES
dc.subject.meshmEpithelial Cellses_ES
dc.subject.meshmKidney-Cytologyes_ES
dc.subject.meshmMelatonines_ES
dc.subject.meshmProtein Kinase Ces_ES
dc.subject.meshmSodium-Potassium-Exchanging ATPasees_ES
dc.subject.meshmStress Fiberses_ES
dc.subject.meshmWater-Metabolismes_ES
dc.subject.meshmWater-Electrolyte Balancees_ES
dc.subject.meshmDrug effectses_ES


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