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Melatonin prevents cytoskeletal alterations and oxidative stress induced by okadaic acid in N1E-115 cells

dc.creatorBenítez-King, G.
dc.creatorTúnez, I.
dc.creatorBellon, A.
dc.creatorOrtíz, G.G.
dc.creatorAntón-Tay, F.
dc.date.accessioned2017-06-29T04:30:03Z
dc.date.available2017-06-29T04:30:03Z
dc.date.issued2003es_ES
dc.identifier392es_ES
dc.identifier.issn0014-4886es_ES
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/5083
dc.identifier.urihttps://doi.org/10.1016/S0014-4886(03)00085-2es_ES
dc.language.isoenges_ES
dc.relation181 (1) 151-159 p.es_ES
dc.relationversión del editores_ES
dc.rightsacceso cerradoes_ES
dc.titleMelatonin prevents cytoskeletal alterations and oxidative stress induced by okadaic acid in N1E-115 cellses_ES
dc.typearticlees_ES
dc.contributor.affiliationInstituto Nacional de Psiquiatría, Departamento de Neurofarmacología, Subdirección de Investigaciones Clínicases_ES
dc.contributor.emailbekin@imp.edu.mxes_ES
dc.relation.jnabreviadoEXP NEUROLes_ES
dc.relation.journalExperimental Neurologyes_ES
dc.identifier.placeEstados Unidoses_ES
dc.date.published2003es_ES
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñizes_ES
dc.description.monthJules_ES
dc.description.abstractotrodiomaProgressive loss of neuronal cytoarchitecture is a major event that precedes neuronal death, both in neural aging and in neurodegenerative diseases. Cytoskeleton in neurodegenerative diseases is characterized by hyperphosphorylated tau assembled in neurofibrillary tangles. Tau protein promotes microtubule enlargement and its hyperphosphorylation inhibits tubulin assembly. Okadaic acid (OA) causes oxidative stress, tau hyperphosphorylation, and altered cytoskeletal organization similar to those observed in neurons of patients with dementia. Since melatonin acts by both enlarging microtubules and as a free-radical scavenger, in this work we studied the effects of melatonin on altered cytoskeletal organization induced by OA in N1E-115 neuroblastoma cells. Optic microscopy, morphometric analysis, and tubulin immunofluorescence staining of neuroblastoma cells incubated with 50 nM OA showed an intact microtubule network following the neurite profile similar to that observed in the vehicle-incubated cells when melatonin was added to the incubation media 2 h before OA. The melatonin effects on altered cytoskeletal organization induced by OA were dose-dependent and were not abolished by luzindole, the mt(1) melatonin antagonist receptor. Also, increased lipid peroxidation and augmented apoptosis in N1E-115 cells incubated with 50 nM OA were prevented by melatonin. The results support the hypothesis that melatonin can be useful in the treatment of neurodegenerative diseases.es_ES
dc.subject.meshmAnimalses_ES
dc.subject.meshmCytoskeleton-Drug effectses_ES
dc.subject.meshmCytoskeleton-Metabolismes_ES
dc.subject.meshmDose-Response Relationshipes_ES
dc.subject.meshmDruges_ES
dc.subject.meshmEnzyme Inhibitors-Pharmacologyes_ES
dc.subject.meshmMelatonin-Pharmacologyes_ES
dc.subject.meshmMicrotubules-Drug effectses_ES
dc.subject.meshmMicrotubules-Metabolismes_ES
dc.subject.meshmNeuroblastoma-Drug therapyes_ES
dc.subject.meshmNeuroblastoma-Metabolismes_ES
dc.subject.meshmOkadaic Acid-Pharmacologyes_ES
dc.subject.meshmOxidative Stress-Physiologyes_ES
dc.subject.meshmPertussis Toxin-Pharmacologyes_ES
dc.subject.meshmPhosphoric Monoester Hydrolases-Antagonists & inhibitorses_ES
dc.subject.meshmTime Factorses_ES
dc.subject.meshmTumor Cellses_ES
dc.subject.meshmCulturedes_ES


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