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Effect of fasting on the content of thyrotropin-releasing hormone and its mRNA in the central nervous system and pyroglutamyl peptidase II activity in the anterior pituitary of post-weaned and adult rats

dc.creatorDe Gortari, P.
dc.creatorGonzález-Alzati, M.E.
dc.creatorCisneros, M.
dc.creatorJoseph-Bravo, P.
dc.date.accessioned2017-06-29T04:24:20Z
dc.date.available2017-06-29T04:24:20Z
dc.date.issued2000es_ES
dc.identifier314es_ES
dc.identifier.issn1028-415Xes_ES
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/5007
dc.identifier.urihttps://doi.org/10.1080/1028415X.2000.11747323es_ES
dc.language.isoenges_ES
dc.relation3 (4) 255-265 p.es_ES
dc.relationversión del editores_ES
dc.rightsacceso cerradoes_ES
dc.titleEffect of fasting on the content of thyrotropin-releasing hormone and its mRNA in the central nervous system and pyroglutamyl peptidase II activity in the anterior pituitary of post-weaned and adult ratses_ES
dc.typearticlees_ES
dc.contributor.affiliationInstituto Mexicano Psiquiatría, México City 14370, DF, México.es_ES
dc.relation.jnabreviadoNUTR NEUROSCIes_ES
dc.relation.journalNutritional Neurosciencees_ES
dc.identifier.placeInglaterraes_ES
dc.date.published2000es_ES
dc.identifier.organizacionInstituto Mexicano de Psiquiatríaes_ES
dc.identifier.eissn1476-8305es_ES
dc.description.abstractotrodiomaFasting and malnutrition affect the hypothalamus-pituitary-thyroid axis causing secondary hypothyroidism. The aim of this study was to determine the effect of 48 h fasting in rats of 4 and 12 weeks old, and of 7 weeks of food restriction, in the activity of adenohypophysial thyrotropin-releasing hormone (TRH)-degrading ectoenzyme; also, to study if these conditions altered peptide levels and its messenger ribonucleic acid (mRNA) in brain areas of the limbic system. Male Wistar rat brains were excised, frozen and dissected for measurement of: (TRH) content by radioimmunoassay, its mRNA by reverse transcriptase-polymerase chain reaction (RT-PCR), the activity of its degrading ectoenzyme, pyroglutamyl peptidase II, by a fluorometric assay, serum thyrotropin and corticosterone by radioimmunoassay. Malnutrition increased median eminence TRH and decreased thyrotropin serum levels as did fasting in adults, that also diminished hypothalamic peptide content; postweaned rats in contrast, only had a decreased pyroglutamyl peptidase II activity in adenohypophysis. Serum corticosterone levels increased in all treatments. Brain TRH content varied differently among groups: a decrease in nucleus (n.) accumbens and an increment in hippocampus of 4 weeks old; a decrease in amygdala of adults (with a concomitant diminution in its mRNA. levels). No variations were observed in food restricted rats. These results show a differential regulation of TRH metabolism by fasting dependent on the age of the animalses_ES
dc.subject.koHypothalamic paraventricular nucleuses_ES
dc.subject.koMessenger-ribonucleic-acides_ES
dc.subject.koThyroid-hormoneses_ES
dc.subject.koDegrading ectoenzymees_ES
dc.subject.koGene-expressiones_ES
dc.subject.koMediobasal hypothalamuses_ES
dc.subject.koAdenohypophyseal cellses_ES
dc.subject.koLuteinizing-hormonees_ES
dc.subject.koInduced suppressiones_ES
dc.subject.koFood-deprivationes_ES


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