Mostrar el registro sencillo del ítem

Acute ethanol administration induces changes in TRH and proenkephalin expression in hypothalamic and limbic regions of rat brain

dc.creatorDe Gortari, P.
dc.creatorMéndez, M.
dc.creatorRodríguez-Keller, I.
dc.creatorPérez-Martínez, L.
dc.creatorJoseph-Bravo, P.
dc.date.accessioned2017-06-29T04:24:12Z
dc.date.available2017-06-29T04:24:12Z
dc.date.issued2000es_ES
dc.identifier312es_ES
dc.identifier.issn0197-0186es_ES
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/5005
dc.identifier.urihttps://doi.org/10.1016/S0197-0186(00)00059-0es_ES
dc.language.isoenges_ES
dc.relation37 (5-6) 483-496 p.es_ES
dc.relationversión del editores_ES
dc.rightsacceso cerradoes_ES
dc.subject.meshAnimalses_ES
dc.subject.meshEnkephalins-Geneticses_ES
dc.subject.meshEnkephalins-Metabolismes_ES
dc.subject.meshEthanol-Pharmacologyes_ES
dc.subject.meshHypothalamus-Metabolismes_ES
dc.subject.meshInjections, Intraperitoneales_ES
dc.subject.meshLimbic System-Metabolismes_ES
dc.subject.meshMalees_ES
dc.subject.meshProtein Precursors-Geneticses_ES
dc.subject.meshProtein Precursors-Metabolismes_ES
dc.subject.meshPyrrolidonecarboxylic Acid-Analogs & derivativeses_ES
dc.subject.meshRNA, Messenger-Metabolismes_ES
dc.subject.meshRatses_ES
dc.subject.meshRats, Wistares_ES
dc.subject.meshThyrotropin-Releasing Hormone-Geneticses_ES
dc.subject.meshThyrotropin-Releasing Hormone-Metabolismes_ES
dc.subject.meshTissue Distributiones_ES
dc.subject.meshEnkephalinses_ES
dc.subject.meshProtein Precursorses_ES
dc.subject.meshRNA, Messengeres_ES
dc.subject.meshProenkephalines_ES
dc.subject.meshThyrotropin-Releasing Hormonees_ES
dc.subject.meshEthanoles_ES
dc.subject.meshPyrrolidonecarboxylic Acides_ES
dc.subject.meshPro-thyrotropin releasing hormonees_ES
dc.titleAcute ethanol administration induces changes in TRH and proenkephalin expression in hypothalamic and limbic regions of rat braines_ES
dc.typearticlees_ES
dc.contributor.affiliationDept. Nutrición, Universidad Iberoamericana, Mexicoes_ES
dc.contributor.emailpgortari@ibt.unam.mxes_ES
dc.relation.jnabreviadoNEUROCHEM INTes_ES
dc.relation.journalNeurochemistry Internationales_ES
dc.identifier.placeInglaterraes_ES
dc.date.published2000es_ES
dc.identifier.organizacionInstituto Mexicano de Psiquiatríaes_ES
dc.identifier.eissn1872-9754es_ES
dc.identifier.doi10.1016/S0197-0186(00)00059-0es_ES
dc.description.monthNoves_ES
dc.description.abstractotrodiomaThyrotropin releasing hormone (TRH) present in several brain areas has been proposed as a neuromodulator. Its administration produces opposite effects to those observed with acute ethanol consumption. Opioid peptides, in contrast, have been proposed to mediate some of the effects of alcohol intoxication. We measured TRH content and the levels of its mRNA in hypothalamic and limbic zones 1–24 h after acute ethanol injection. We report here fast and transient changes in the content of TRH and its mRNA in these areas. The levels of proenkephalin mRNA varied differently from those of proTRH mRNA, depending on the time and region studied. Wistar rats were administered one dose of ethanol (intraperitoneal, 3 g-kg body weight) and brains dissected in hypothalamus, hippocampus, amygdala, n. accumbens and frontal cortex, for TRH quantification by radioimmunoassay or for proTRH mRNA measurement by RT-PCR. After 1 h injection, TRH levels were increased in hippocampus and decreased in n. accumbens; after 4 h, it decreased in the hypothalamus, frontal cortex and amygdala, recovering to control values in all regions at 24 h. ProTRH mRNA levels increased at 1 h post-injection in total hypothalamus and hippocampus, while they decreased in the frontal cortex. The effect of ethanol was also studied in primary culture of hypothalamic cells; a fast and transient increase in proTRH mRNA was observed at 1 h of incubation (0.001% final ethanol concentration). Changes in the mRNA levels of proTRH and proenkephalin were quantified by in situ hybridization in rats administered ethanol intragastrically (2.5 g-kg). Opposite alterations were observed for these two mRNAs in hippocampus and frontal cortex, while in n. accumbens and the paraventricular nucleus of the hypothalamus, both mRNA levels were increased but with different kinetics. These results give support for TRH and enkephalin neurons as targets of ethanol and, as possible mediators of some of its observed behavioral effectses_ES


Ficheros en el ítem

FicherosTamañoFormatoVer

No hay ficheros asociados a este ítem.

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem