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Three TRH-like molecules are released from rat hypothalamus in vitro

dc.creatorMéndez, M
dc.creatorCisneros, M
dc.creatorBaez, A
dc.creatorJoseph-Bravo, P
dc.creatorCharli, JL.
dc.date.accessioned2017-06-29T04:22:39Z
dc.date.available2017-06-29T04:22:39Z
dc.date.issued1999es_ES
dc.identifier285es_ES
dc.identifier.issn0364-3190es_ES
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/4979
dc.identifier.urihttps://doi.org/10.1023/A:1020993527602es_ES
dc.language.isoenges_ES
dc.relation24 (7) 815-823 p.es_ES
dc.relationversión del editores_ES
dc.rightsacceso cerradoes_ES
dc.titleThree TRH-like molecules are released from rat hypothalamus in vitroes_ES
dc.typearticlees_ES
dc.contributor.affiliationDepartamento de Genética y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, MEXICOes_ES
dc.relation.jnabreviadoNEUROCHEM RESes_ES
dc.relation.journalNeurochemical Researches_ES
dc.identifier.placeUnited Stateses_ES
dc.date.published1999es_ES
dc.identifier.organizacionInstituto Mexicano de Psiquiatríaes_ES
dc.description.monthJules_ES
dc.description.abstractotrodiomaTRH-like immunoreactivity distinct from TRH is present in various tissues and fluids. In order to determine whether TRH-like molecules are secreted by the hypothalamus, we analyzed tissues and media from hypothalamic slices incubated in Krebs Ringer bicarbonate. Media from basal or high KCl conditions contained 3 TRH-like molecules evidenced by reverse phase high performance liquid chromatography followed by TRH radioimmunoassay. Peak I corresponded to authentic TRH (73% of total immunoreactivity) and peaks II and III had a higher retention time. These additional TRH-like forms were neither detected in hypothalamic tissue nor in tissue or medium from olfactory bulb. Gel filtration analysis of hypothalamic media revealed only one TRH-like peak eluting as TRH, suggesting that the molecular weights of peaks II and III are similar to that of TRH. Peak II retention time was similar to that of pglu-phe-proNH2. We analysed if they could be produced by post secretory metabolism of TRH. Incubation of hypothalamic slices with [3H-Pro]-TRH did not produce radioactive species comigrating with peaks II or III. However, it induced rapid degradation to [3H-Pro]-his-prodiketopiperazine ([3H]-HPDKP). Inhibitor profile suggested that pyroglutamyl aminopeptidase II, but not pyroglutamyl aminopeptidase I, is responsible for [3H]-HPDKP production. These data are consistent with the hypothesis that pyroglutamyl aminopeptidase II is the main aminopeptidase degrading TRH in hypothalamic extracellular fluid. Furthermore, we suggest that the hypothalamus releases additional TRH-like molecules, one of them possibly pglu-phe-proNH2, which may participate in control of adenohypophyseal secretions.es_ES


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