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Cell volume changes upon sodium pump inhibition in Helix aspersa neurones

dc.creatorAlvarez-Leefmans, F.J.
dc.creatorGamiño, S.M.
dc.creatorReuss, L.
dc.date.accessioned2017-06-29T04:16:14Z
dc.date.available2017-06-29T04:16:14Z
dc.date.issued1991es_ES
dc.identifier149es_ES
dc.identifier.issn0022-3751es_ES
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/4843
dc.identifier.urihttps://doi.org/10.1113/jphysiol.1992.sp019436es_ES
dc.language.isoenges_ES
dc.relation458 (1) 603-619 p.es_ES
dc.relationversión del editores_ES
dc.rightsacceso cerradoes_ES
dc.titleCell volume changes upon sodium pump inhibition in Helix aspersa neuroneses_ES
dc.typearticlees_ES
dc.contributor.affiliationDepartamento de Farmacología y Toxicología, Centro de Investigación y de Estudios Avanzados del IPN, México, D.F.es_ES
dc.relation.jnabreviadoJ PHYSIOL (LOND)es_ES
dc.relation.journalThe Journal of physiologyes_ES
dc.identifier.placeInglaterraes_ES
dc.date.published1992es_ES
dc.identifier.organizacionInstituto Mexicano de Psiquiatríaes_ES
dc.identifier.eissn1469-7793es_ES
dc.description.monthDices_ES
dc.description.abstractotrodioma1. Identified neurones of the suboesophageal ganglia of Helix aspersa were loaded with tetramethylammonium (TMA+). Experimentally induced changes in cell water volume and membrane potential were measured continuously by monitoring changes in intracellular [TMA+] using ion-sensitive double-barrelled microelectrodes. The technique allowed measurements of cell water volume changes of less than 5%. 2. Exposure to hyperosmotic (up to +24%) or hyposmotic (up to about -10%) solutions caused reversible decreases and increases in cell water volume respectively, which agreed with near-ideal osmometric behaviour. Upon exposure to hyposmotic solutions whose osmolality was decreased by 30-40%, the cell water volume increased to maximum values below those expected for ideal osmometric behaviour and exhibited partial regulatory volume decrease. 3. The sodium pump was inhibited in twenty identified neurones by sustained exposure to 1 mM ouabain. In every case ouabain caused cell membrane depolarization, as expected for inhibition of an electrogenic sodium pump. 4. Upon pump inhibition most cells (n = 14) shrank by up to 13% of their initial water volume. In five of these cells, shrinkage was preceded by one or more short-lived swelling phases. In two other neurones short-lived swelling was followed by cell volume recovery without appreciable shrinkage. In four out of the twenty cells, there were no measurable volume changes. 5. The lack of an initial swelling phase in the cells that shrank, as well as the absence of detectable volume changes in some of the neurones, was not due to loss of ion-selective electrode sensitivity since predictable changes in cell volume elicited by osmotic challenges were monitored in the same cells. 6. It is concluded that neurones can be endowed with ouabain-insensitive mechanisms of volume control, whose activation following Na+ pump inhibition prevents them from short-term swelling and lysis.es_ES
dc.subject.koAnimalses_ES
dc.subject.koCell Sizees_ES
dc.subject.koHelix (Snails)-Physiologyes_ES
dc.subject.koMembrane Potentials-Drug effectses_ES
dc.subject.koNeurons-Cytologyes_ES
dc.subject.koNeurons-Metabolismes_ES
dc.subject.koOsmolar Concentrationes_ES
dc.subject.koQuaternary Ammonium Compounds-Pharmacologyes_ES
dc.subject.koSodium-Potassium-Exchanging ATPase-Drug effectses_ES
dc.subject.koSodium-Potassium-Exchanging ATPase-Physiologyes_ES
dc.subject.koQuaternary Ammonium Compoundses_ES
dc.subject.koTetramethylammoniumes_ES
dc.subject.koSodium-Potassium-Exchanging ATPasees_ES


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