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Melatonin effects on the cytoskeletal organization of MDCK and neuroblastoma N1E-115 cells

dc.creatorBenítez-King, G.
dc.creatorHuerto-Delgadillo, L.
dc.creatorAntón-Tay, F.
dc.date.accessioned2017-06-29T04:14:41Z
dc.date.available2017-06-29T04:14:41Z
dc.date.issued1990es_ES
dc.identifier97es_ES
dc.identifier.issn0742-3098es_ES
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/4792
dc.identifier.urihttps://doi.org/10.1111/j.1600-079X.1990.tb00709.xes_ES
dc.language.isoenges_ES
dc.relation9 (3) 209-220 p.es_ES
dc.relationversión del editores_ES
dc.rightsacceso cerradoes_ES
dc.titleMelatonin effects on the cytoskeletal organization of MDCK and neuroblastoma N1E-115 cellses_ES
dc.typearticlees_ES
dc.contributor.affiliationDepartmento de Neurofarmacología, Instituto Mexicano de Psiquiatría Mexicoes_ES
dc.relation.jnabreviadoJ PINEAL RESes_ES
dc.relation.journalJournal of Pineal Researches_ES
dc.identifier.placeDenmarkes_ES
dc.date.published1990es_ES
dc.identifier.organizacionInstituto Mexicano de Psiquiatríaes_ES
dc.description.abstractotrodiomaDespite the fact that many physiological and pharmacological actions of melatonin (MEL) have been described, its mechanism of action at the subcellular level remains unclear. It has been suggested that MEL has effects on cellular processes that involve microfilaments and microtubules. In the present study MEL effects on the cytoskeleton were evaluated in MDCK and N1E-115 cells in which the microfilaments have been shown to participate in cell morphology and dome formation (MDCK) and the microtubules in neurite outgrowths. After one day of culture with 10(-11)-10(-7) M MEL MDCK cells showed an increase in the number of elongated cells. After four days with the hormone, an increase in the incidence of MDCK cells contacting neighboring cells through long cytoplasmic elongations was observed. Actin antibody stain showed the appearance of thicker fluorescent fibres beneath the cell membrane and over the nucleus in the MEL treated cells. An increase in dome formation in confluent cells was also observed. In N1E-115 cells MEL (10(-13)-10(-5) M) induced an increase in cell with neurite processes. Neurite outgrowth is clearly seen at 24 h after plating. MEL-treated cells grow in clusters with neurites forming intricate networks. Antitubulin antibody stain showed long fluorescent neurites in the N1E-115 MEL-treated cells. A decrease in N1E-115 neurite formation was observed with either serotonin or 6-hydroxymelatonin (6OH-MEL). However, the number of MDCK cells with cytoplasmic elongations was decreased only after 6OH-MEL. We conclude that MEL action at the cellular level involves a modification of the cytoskeletal organization.es_ES


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