Mostrar el registro sencillo del ítem

Synthesis and phosphorylation of cytoskeletal proteins during the in vitro biogenesis of MDCK cell monolayers

dc.creatorBenítez-King, G.
dc.creatorCazares, F.
dc.creatorMeza, I.
dc.date.accessioned2017-06-29T04:14:21Z
dc.date.available2017-06-29T04:14:21Z
dc.date.issued1989es_ES
dc.identifier83es_ES
dc.identifier.issn0021-9533es_ES
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/4778
dc.language.isoenges_ES
dc.relation93 (1) 53-61 p.es_ES
dc.relationversión del editores_ES
dc.rightsacceso cerradoes_ES
dc.titleSynthesis and phosphorylation of cytoskeletal proteins during the in vitro biogenesis of MDCK cell monolayerses_ES
dc.typearticlees_ES
dc.contributor.affiliationDepto de Biología Celular, Centro de Investigación y de Estudios Avanzados, México, D.Fes_ES
dc.relation.jnabreviadoJ CELLSCIes_ES
dc.relation.journalJournal Of Cell Sciencees_ES
dc.identifier.placeInglaterraes_ES
dc.date.published1989es_ES
dc.identifier.organizacionInstituto Mexicano de Psiquiatríaes_ES
dc.description.monthMayes_ES
dc.description.abstractotrodiomaIn vitro formation of a functional MDCK cell monolayer requires the dynamic participation of the cytoskeleton. Cell shape, contacts and polarity, as well as transepithelial electric resistance (TER), are actively modified during this differentiation process. We studied the distribution and rearrangement of cytokeratin, vimentin and actin filaments that occur in the monolayer concomitant with the synthesis and phosphorylation of these proteins. Cells cultured for short time in suspension, subconfluent or early confluent cells, showed striking differences in cell shape and arrangement of their cytoskeletal filaments. Subconfluent and early confluent cells synthesized proteins at high levels. In contrast, suspended cells maintained lower rates of protein synthesis. Labeled amino acid uptake was very similar in all these culture conditions. Gel electrophoresis analyses of the synthesized proteins showed increases in the synthesis of actin, vimentin and specific cytokeratins in subconfluent and early confluent cells. On the other hand a decrease in total protein, actin, vimentin and cytokeratin synthesis was observed in cells kept in suspension for 24 h or in 78-h or 174-h older confluent cultures. These cultures also showed a decrease in the uptake of labeled amino acid. Cytokeratin and vimentin phosphorylation rates were also modified during the in vitro formation of a monolayer. In suspended cells, cytokeratins were phosphorylated and three labeled isoelectric variants of the 40, 48 and 58K (K = 10(3) Mr) cytokeratins were present in intermediate filament extracts. In subconfluent and early confluent cells only two isoelectric phosphorylated variants of the 40, 48 and 58K cytokeratins were detected and vimentin was also phosphorylated.(ABSTRACT TRUNCATED AT 250 WORDS)es_ES


Ficheros en el ítem

FicherosTamañoFormatoVer

No hay ficheros asociados a este ítem.

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem