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Molecular identity, ontogeny, and cAMP modulation of the hyperpolarizationactivated current in vestibular ganglion neurons

dc.creatorAlmanza, Angélica
dc.creatorLuis, Enoch
dc.creatorMercado, Francisco
dc.creatorVega, Rosario
dc.creatorSoto, Enrique
dc.date.accessioned2017-06-29T04:00:14Z
dc.date.available2017-06-29T04:00:14Z
dc.date.issued2012es_ES
dc.identifier2835es_ES
dc.identifier.issn0022-3077es_ES
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/4684
dc.identifier.urihttps://doi.org/10.1152/jn.00337.2012es_ES
dc.description.abstractes_ES
dc.language.isoenges_ES
dc.publisherBethesda Md : American Physiological Societyes_ES
dc.relation108 (8) 2264-2275 p.es_ES
dc.relationversión del editores_ES
dc.rightsacceso cerradoes_ES
dc.subject.meshAction Potentials-Drug effectses_ES
dc.subject.meshAction Potentials-Physiologyes_ES
dc.subject.meshAnimalses_ES
dc.subject.meshCyclic AMP-Metabolismes_ES
dc.subject.meshCyclic Nucleotide-Gated Cation Channels-Geneticses_ES
dc.subject.meshCyclic Nucleotide-Gated Cation Channels-Metabolismes_ES
dc.subject.meshCyclic Nucleotide-Gated Cation Channels-Physiologyes_ES
dc.subject.meshGene Expression Regulation, Developmentales_ES
dc.subject.meshNeurons-Metabolismes_ES
dc.subject.meshNeurons-Physiologyes_ES
dc.subject.meshPotassium Channel Blockers-Pharmacologyes_ES
dc.subject.meshProtein Isoforms-Geneticses_ES
dc.subject.meshProtein Isoforms-Metabolismes_ES
dc.subject.meshProtein Isoforms-Physiologyes_ES
dc.subject.meshProtein Subunits-Geneticses_ES
dc.subject.meshProtein Subunits-Metabolismes_ES
dc.subject.meshProtein Subunits-Physiologyes_ES
dc.subject.meshRatses_ES
dc.subject.meshRats, Long-Evanses_ES
dc.subject.meshVestibular Nuclei-Cytologyes_ES
dc.subject.meshVestibulares_ES
dc.subject.meshNuclei-Growth & developmentes_ES
dc.titleMolecular identity, ontogeny, and cAMP modulation of the hyperpolarizationactivated current in vestibular ganglion neuronses_ES
dc.title.alternativees_ES
dc.typeartículoes_ES
dc.contributor.affiliationInstituto de Fisiologíaes_ES
dc.contributor.emailesoto24@gmail.comes_ES
dc.relation.jnabreviadoJ NEUROPHYSIOLes_ES
dc.relation.journalJournal of Neurophysiologyes_ES
dc.identifier.placeEstados Unidoses_ES
dc.date.published2012es_ES
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñizes_ES
dc.identifier.eissn1522-1598es_ES
dc.identifier.doi10.1152/jn.00337.2012es_ES
dc.description.monthOctes_ES
dc.description.abstractotrodiomaProperties, developmental regulation, and cAMP modulation of the hyperpolarization-activated current (Ih) were investigated by the whole cell patch-clamp technique in vestibular ganglion neurons of the rat at two postnatal stages (P7–10 and P25–28). In addition, by RT-PCR and immunohistochemistry the identity and distribution of hyperpolarization-activated and cyclic nucleotide-gated channel (HCN) isoforms that generate Ih were investigated. Ih current density was larger in P25–28 than P7–10 rats, increasing 410% for small cells ( 30 pF) and 200% for larger cells ( 30 pF). The half-maximum activation voltage (V1/2) of Ih was 102 mV in P7–10 rats and in P25–28 rats shifted 7 mV toward positive voltages. At both ages, intracellular cAMP increased Ih current density, decreased its activation time constant ( ), and resulted in a rightward shift of V1/2 by 9 mV. Perfusion of 8-BrcAMP increased Ih amplitude and speed up its activation kinetics. Ih was blocked by Cs , zatebradine, and ZD7288. As expected, these drugs also reduced the voltage sag caused with hyperpolarizing pulses and prevented the postpulse action potential generation without changes in the resting potential. RT-PCR analysis showed that HCN1 and HCN2 subunits were predominantly amplified in vestibular ganglia and end organs and HCN3 and HCN4 to a lesser extent. Immunohistochemistry showed that the four HCN subunits were differentially expressed (HCN1 HCN2 HCN3 HCN4) in ganglion slices and in cultured neurons at both P7–10 and P25–28 stages. Developmental changes shifted V1/2 of Ih closer to the resting membrane potential, increasing its functional role. Modulation of Ih by cAMP-mediated signaling pathway constitutes a potentially relevant control mechanism for the modulation of afferent neuron discharge.es_ES
dc.subject.meshmes_ES
dc.subject.kwes_ES
dc.subject.koInner eares_ES
dc.subject.koIhes_ES
dc.subject.kohair celles_ES
dc.subject.koZatebradinees_ES
dc.subject.koZD7288es_ES
dc.subject.koCampes_ES


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