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Interferon-_ 2b quantification in inclusion bodies using Reversed Phase-Ultra Performance Liquid Chromatography (RP-UPLC)

dc.creatorCueto-Rojas, H.F.
dc.creatorPérez, N.O.
dc.creatorPérez-Sánchez, G.
dc.creatorOcampo-Juárez, I.
dc.creatorMedina-Rivero, E.
dc.date.accessioned2017-06-29T03:54:23Z
dc.date.available2017-06-29T03:54:23Z
dc.date.issued2010es_ES
dc.identifier2773es_ES
dc.identifier.issn1572-6495es_ES
dc.identifier.urihttp://dx.doi.org/10.1016/j.jchromb.2010.02.016es_ES
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/4622
dc.language.isoenges_ES
dc.publisherAmsterdam, Netherlands : Elsevieres_ES
dc.relation878 (13-14) 1019-1023 p.es_ES
dc.relationversión del editores_ES
dc.rightsacceso cerradoes_ES
dc.titleInterferon-_ 2b quantification in inclusion bodies using Reversed Phase-Ultra Performance Liquid Chromatography (RP-UPLC)es_ES
dc.typeartículoes_ES
dc.contributor.affiliationProbiomed S.A. de C.V., Departamento de Investigación y Desarrollo, Cruce de carreteras Acatzingo-Zumpahuacán S/N, Tenancingo, Estado de México, México 52400, Mexicoes_ES
dc.contributor.emailemilio.medina@probiomed.com.mxes_ES
dc.relation.jnabreviadoJ CHROMATOGR B BIOMED APPLes_ES
dc.relation.journalJournal of Chromatography. B, Biomedical applicationses_ES
dc.identifier.placePaíses Bajoses_ES
dc.date.published2010es_ES
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñizes_ES
dc.identifier.doi10.1016/j.jchromb.2010.02.016es_ES
dc.description.monthAbres_ES
dc.description.abstractotrodiomaInterferon-_ 2b (IFN-_ 2b) is a recombinant therapeutic cytokine produced as inclusion bodies using a strain of Escherichia coli as expression system. After fermentation and recovery, it is necessary to know the amount of recombinant IFN-_ 2b, in order to determine the yield and the load for solubilization, and chromatographic protein purification steps. The present work details the validation of a new short run-time and fast sample-preparation method to quantify IFN-_ 2b in inclusion bodies using Reversed Phase-Ultra Performance Liquid Chromatography (RP-UPLC). The developed method demonstrated an accuracy of 100.28%; the relative standard deviations for method precision, repeatability and inter-day precision tests were found to be 0.57%, 1.54% and 1.83%, respectively. Linearity of the method was assessed in the range of concentrations from 0.05mg/mL to 0.5 mg/mL, the curve obtained had a determination coefficient (r2) of 0.9989. Detection and quantification limits were found to be 0.008mg/mL and 0.025 mg/mL, respectively. The method also demonstrated robustness for changes in column temperature, and specificity against host proteins and other recombinant protein expressed in the same E. coli straines_ES
dc.subject.koInterferon-_ 2bes_ES
dc.subject.koUPLCes_ES
dc.subject.koValidationes_ES
dc.subject.koInclusion bodyes_ES


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