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Cycling of dense core vesicles involved in somatic exocytosis of serotonin by leech neurons

dc.creatorTrueta, Citlali
dc.creatorKuffler, Damien P.
dc.creatorDe-Miguel, Francisco F.
dc.date.accessioned2017-06-29T03:44:21Z
dc.date.available2017-06-29T03:44:21Z
dc.date.issued2012es_ES
dc.identifier2621es_ES
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/4470
dc.identifier.urihttps://doi.org/10.3389/fphys.2012.00175es_ES
dc.language.isoenges_ES
dc.publisherFrontiers Media S.Aes_ES
dc.relation3(175) 1-13p.es_ES
dc.relationversión del editores_ES
dc.rightsacceso cerradoes_ES
dc.titleCycling of dense core vesicles involved in somatic exocytosis of serotonin by leech neuronses_ES
dc.typeartículoes_ES
dc.contributor.affiliationInstituto Nacional de Psiquiatría “Ramón de la Fuente Muñiz", MéxicoD.F.,Méxicoes_ES
dc.contributor.emailffernand@ifc.unam.mxes_ES
dc.relation.jnabreviadoFRONT PHYSIOLes_ES
dc.relation.journalFrontiers in Physiologyes_ES
dc.identifier.placeSwitzerlandes_ES
dc.date.published2012es_ES
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñizes_ES
dc.identifier.eissn1664-042Xes_ES
dc.identifier.doi10.3389/fphys.2012.00175es_ES
dc.description.monthJunes_ES
dc.description.abstractotrodiomaWestudiedthecyclingofdensecorevesiclesproducingsomaticexocytosisofserotonin.Ourexperimentsweremadeusingelectronmicroscopyandvesiclestainingwithfluores-centdyeFM1-43inRetziusneuronsoftheleech,whichsecreteserotoninfromclustersofdensecorevesiclesinafrequency-dependentmanner.Electronmicrographsofneuronsatrestorafter1Hzstimulationshowedtwopoolsofdensecorevesicles.AperinuclearpoolnearGolgiapparatuses,fromwhichvesiclesapparentlyform,andaperipheralpoolwithvesicleclustersatadistancefromtheplasmamembrane.Bycontrast,after20Hzelectricalstimulation47%ofthevesicleclusterswereapposedtotheplasmamembrane,withsomeomegaexocytosisstructures.Densecoreandsmallclearvesiclesapparentlyoriginatingfromendocytosiswereincorporatedinmultivesicularbodies.Inanotherseriesofexperiments,neuronswerestimulatedat20Hzwhilebathedinasolutioncontainingperoxidase.Electronmicrographsoftheseneuronscontainedgoldparticlescoupledtoanti-peroxidaseantibodiesindensecorevesiclesandmultivesicularbodieslocatedneartheplasmamembrane.Culturedneuronsdepolarizedwithhighpotassiuminthepres-enceofFM1-43displayedsuperficialfluorescentspots,eachreflectingavesiclecluster.ApartialbleachingofthespotsfollowedbyanotherdepolarizationinthepresenceofFM1-43producedrestainingofsomespots,otherspotsdisappeared,someremainedwithoutrestainingandnewspotswereformed.SeveralhoursafterelectricalstimulationtheFM1-43spotsaccumulatedatthecenterofthesomata.ThiscorrelatedwithelectronmicrographsofmultivesicularbodiesreleasingtheircontentsnearGolgiapparatuses.Ourresultssuggestthatdensecorevesiclecyclingrelatedtosomaticserotoninreleaseinvolvestwosteps:theproductionofclearvesiclesandmultivesicularbodiesafterexocytosis,andtheformationofnewdensecorevesiclesintheperinuclearregion.es_ES
dc.subject.koSerotonines_ES
dc.subject.koSomaticexocytosises_ES
dc.subject.koExtrasynapticexocytosises_ES
dc.subject.koDensecorevesiclees_ES
dc.subject.koVesiclecyclees_ES
dc.subject.koVolumees_ES
dc.subject.koTransmissiones_ES
dc.subject.koEndocytosises_ES
dc.subject.koLeeches_ES


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